NDLI: Availability of genetically modified feed ingredient: investigations of ingested foreign DNA in rainbow trout Oncorhynchus mykiss

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Fisheries Science : Volume 74, Issue 1, January 2008

Fisheries Science : Volume 73

Fisheries Science : Volume 72

Fisheries Science : Volume 71

Availability of genetically modified feed ingredient: investigations of ingested foreign DNA in rainbow trout Oncorhynchus mykiss

Content Provider	Springer Nature Link
Author	Chainark, Pitchaya Satoh, Shuichi Hirono, Ikuo Aoki, Takashi Endo, Makoto
Copyright Year	2008
Abstract	Foreign DNA fragments from genetically modified defatted soybean meal (GM SEM) in rainbow trout was traced by nested polymerase chain reaction (PCR) and located by in situ hybridization. Either a GM or non-GM SBM formulated diet (42% protein) was fed to fish (average weight 50.5 g) for 2 weeks. The degradation results showed that the cauliflower mosaic virus 35S promoter (220 bp) fragment was detected in the contents of digestive system only in fish fed the GM SBM diet, and it was not detected on the third day after changing the diet to the non-GM SBM diet. For the possible transferal results, the promoter fragment was detected in the leukocyte, head kidney and muscle only of fish fed the GM SBM diet; it was not detected on the fifth day after changing the diet to the non-GM SBM diet. These results suggest that a foreign DNA fragment was not completely degraded and might be taken up into organs through the gastrointestinal tract. However, foreign DNA was not detected after the withdrawal period. Thus, the data show that uptake of DNA from GM SBM might not remain in the tissues of fish fed GM SBM diet.
Starting Page	380
Ending Page	390
Page Count	11
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	74
Issue Number	2
e-ISSN	14442906
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	CaMV 35S promoter gastrointestinal tract genetically modified defatted soybean meal in situ hybridization Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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Availability of genetically modified soybean meal in rainbow trout Oncorhynchus mykiss diets

Supplemental effect of bile salts to soybean meal-based diet on growth and feed utilization of rainbow trout Oncorhynchus mykiss

Effect of replacing fish meal with soybean meal on growth, feed utilization and nitrogen and phosphorus excretion on rainbow trout (Oncorhynchus mykiss)

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Supplemental effect of cholyltaurine and soybean lecithin to a soybean meal-based fish meal-free diet on hepatic and intestinal morphology of rainbow trout Oncorhynchus mykiss

Optimization of the supplemental essential amino acids to a fish meal-free diet based on fermented soybean meal for rainbow trout Oncorhynchus mykiss

Bioprocessed soybean meal replacement of fish meal in rainbow trout (Oncorhynchus mykiss) diets

Suitability of genetically modified soybean meal in a dietary ingredient for common carp Cyprinus carpio

The Use of Hazelnut Meal as a Substitute for Soybean Meal in the Diets of Rainbow Trout (Oncorhynchus mykiss)

Availability of genetically modified feed ingredient: investigations of ingested foreign DNA in rainbow trout Oncorhynchus mykiss

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