NDLI: Evaluation of genetic diversity of eight grouper species Epinephelus spp. based on microsatellite variations

Content Provider	Springer Nature Link
Author	Koedprang, Worawut Na Nakorn, Uthairat Nakajima, Masamichi Taniguchi, Nobuhiko
Copyright Year	2007
Abstract	Genetic diversity among eight species of grouper, Epinephelus bleekeri, E. coioides, E. malabaricus, E. ongus, E. akaara, E. maculatus, E. merra and E. fuscoguttatus, was studied using six microsatellite loci, Em-01 $^{⋆}$, Em-03 $^{⋆}$, Em-07 $^{⋆}$, Em-08 $^{⋆}$, Em-10 $^{⋆}$, and CA-07 $^{⋆}$, with the aim of exploring the feasibility of using microsatellite data for species identification. The results showed high levels of genetic differentiation among species (F $_{ st }$=0.4403 and R $_{ st }$=0.4954). Species identification based on fixed allelic differences was possible between E. coioides, E. ongus, and E. fuscoguttatus at Em-01 $^{⋆}$ and between E. fuscoguttatus and E. ongus at Em-08 $^{⋆}$. Private alleles were found in all species, except for E. ongus. Pairwise F $_{ st }$ ranged 0.238–0.578 (P<0.008 Bonferroni correction), and Nei’s genetic distance ranged 0.433–2.710. Size homoplasy was observed at Em-03 $^{⋆}$ 157 allele, which was characterized by a T-C transition at the 119th nucleotide site of PCR products. The genetic assignment test unambiguously assigned each individual to the correct species. Thus, this test can be used for species identification of unknown individuals when the multilocus genotypes of the six microsatellite loci are available. The phylogenetic (neighbor-joining) tree, which was constructed based on the genetic distance matrix, separated the eight grouper species into two main groups.
Starting Page	227
Ending Page	236
Page Count	10
File Format	PDF
ISSN	09199268
Journal	Fisheries Science
Volume Number	73
Issue Number	2
e-ISSN	14442906
Language	English
Publisher	Springer-Verlag
Publisher Date	2007-01-01
Publisher Place	Tokyo
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Epinephelus genetic diversity grouper species homoplasy microsatellite Fish & Wildlife Biology & Management Freshwater & Marine Ecology Food Science
Content Type	Text
Resource Type	Article
Subject	Aquatic Science

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