NDLI: Flavonoid Production Is Effectively Regulated by RNAi Interference of Two Flavone Synthase Genes from Glycine max

Content Provider	Springer Nature Link
Author	Jiang, Yi Na Wang, Biao Li, Hui Yao, Lu Ming Wu, Tian Long
Copyright Year	2010
Abstract	Flavonoids are a group of secondary metabolites found in many higher plants. The multiple roles of their flavone subclass include protection against UV damage, regulation of auxin transport, and modulation of flower color. In soybean (Glycine max), flavone synthase II (FNS II) is the key enzyme responsible for flavone biosynthesis. Two FNS II genes from soybean cultivar Hefeng 47 were cloned according to basic local alignment search tool (BLAST) contexts using flavone synthase sequences reported in other species. These were named GmFNSII-1 and GmFNSII-2. Sequence alignments showed that the cDNA of GmFNSII-1 was identical to that of CYP93B16, whereas GmFNSII-2 was clearly distinct. Functional assays in yeast (Schizosaccharomyces pombe) suggested that these two enzymes could convert (2S)-naringenin into apigenin. The two GmFNSII genes had similar tissue-specific expression patterns, but GmFNSII-2 was significantly expressed in the roots after treatment with 0.4 M glucose. This demonstrates that the gene plays an important role in the response to defense signals in soybean. RNA interference-mediated suppression of those GmFNSII genes effectively regulated flavone and isoflavone production in hairy roots that arose from soybean cotyledons transformed with Agrobacterium rhizogenes (ATCC15834). Our study also highlights some of the challenges associated with metabolic engineering of plant natural products.
Starting Page	425
Ending Page	432
Page Count	8
File Format	PDF
ISSN	12269239
Journal	Journal of Plant Biology
Volume Number	53
Issue Number	6
e-ISSN	18670725
Language	English
Publisher	Springer-Verlag
Publisher Date	2010-11-03
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Flavones Flavone synthase II Isoflavones Soybean Subcellular localization Plant Ecology Plant Systematics/Taxonomy/Biogeography Plant Genetics & Genomics Plant Breeding/Biotechnology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science

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