NDLI: Transformation of riceOsMADS1 gene causes homeotic mutations in floral organs of chinese cabbage (Brassica campestris)

Content Provider	Springer Nature Link
Author	Shin, Junhye Cho, Hongjoo Park, Min Chul Chung, Yong Yoon
Copyright Year	2003
Abstract	We introducedOsMADS1, a MADS box gene from rice, into Chinese cabbage using theAgrobacterium- mediated method. Among the 30 regenerated transformants, 7 transgenic plants were confirmed to carryOsMADS1 transcript as the transgene. This gene had been put under the 35S promoter by RT-PCR and RT-PCR northern hybridization. TheNPT II andOsMADS1 primers detected 0.7-kb and 0.6-kb PCR products, respectively, that corresponded with the expected size from their transcripts. Our results suggest that those transgenes are expressed in the transgenic plants. We also observed the plants at the flowering stage in order to identify any possible morphological alteration caused by the transgene during flowering. One notable mutation involved the homeotic replacement of a carpel with another flower instead.
Starting Page	46
Ending Page	51
Page Count	6
File Format	PDF
ISSN	12269239
Journal	Journal of Plant Biology
Volume Number	46
Issue Number	1
e-ISSN	18670725
Language	English
Publisher	Springer-Verlag
Publisher Date	2003-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Chinese cabbage flower development homeotic mutation OsMADS1 Plant Sciences Plant Breeding/Biotechnology Plant Genetics & Genomics Plant Systematics/Taxonomy/Biogeography Plant Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

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Transformation of riceOsMADS1 gene causes homeotic mutations in floral organs of chinese cabbage (Brassica campestris)