NDLI: Expression analysis of the metacaspase gene family in Arabidopsis

Content Provider	Springer Nature Link
Author	Kwon, Soon Il Hwang, Duk Ju
Copyright Year	2013
Abstract	Metacaspase is discovered gene family with distant caspase homologs in protozoa, fungi, and plants. The Arabidopsis genome has nine metacaspase genes. Based on the presence or absence of an N-terminal domain, they can be divided into two types of metacaspase genes: type I (AtMC1 to 3) and type II (AtMC4 to 9). To examine the expression patterns of the Arabidopsis metacaspase gene family, we measured the levels of expression of the metacaspase genes in various tissues and during biotic stresses by quantitative reverse transcription-polymerase chain reaction. As expected, the nine AtMC genes were expressed differently among tissues and during diverse stress conditions. In addition, to address the spatial expression of AtMC genes, their expression patterns using the promoter region of AtMCs (0.85 kb to 2.55 kb) were examined by the GUS reporter system. By observing GUS activity in transgenic plants containing nine AtMCpro::GUS constructs, we found that expression of these nine AtMC genes was regulated developmentally and spatially. We also observed that AtMC::green fluorescent fusion proteins were mainly localized in the nucleus using the Arabidopsis protoplast transient assay. In summary, our data suggest that AtMCs gene family be involved in various defense responses during biotic stresses.
Starting Page	391
Ending Page	398
Page Count	8
File Format	PDF
ISSN	12269239
Journal	Journal of Plant Biology
Volume Number	56
Issue Number	6
e-ISSN	18670725
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2013-11-12
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cell death FB1 Flg22 Metacaspase Plant Sciences Plant Breeding/Biotechnology Plant Genetics & Genomics Plant Systematics/Taxonomy/Biogeography Plant Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

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