NDLI: A Simple and Non-destructive Method of Direct-PCR for Plant Systems

Content Provider	Springer Nature Link
Author	Sharma, R. Kumar, Vinod Mohapatra, T. Khandelwal, Vikas Vyas, Govind K.
Copyright Year	2011
Abstract	To date, PCR is a fundamental tool for most of the research concerning plant diversity analysis, marker-assisted selection, genetic purity testing, disease diagnostics, and transgene analysis. In all of these analyses, good-quality DNA serves as a template for amplification of target sequences. Extraction of good-quality DNA requires many steps, making the whole process time consuming, tedious, labor intensive, and expensive due to costlier and toxic chemicals. To overcome these preparatory steps from PCR-based DNA amplification, we have developed a direct-PCR amplification method for plants without isolating DNA. The method is unique and beneficial over some previously described methods of direct-PCR which fail due to inefficient amplification of target DNA in the presence of PCR inhibitors and crop specificity. Moreover, such methods are non-specific and, being destructive, cannot be replicated; one cannot completely rely on them due to lack of reproducibility. This method was streamlined from our earlier observation that alcohol-desiccated tissues maintain intact DNA for a long time. This method is specific, rapid, and, being non-destructive, allows replication, giving advantages over existing methods. The method was tested over a wide range of plant species and found very effective and quick in generating data. The method was successfully used to test the genetic purity of pearl millet hybrid (RHB-127) and its restorer (RIB 3135-18) and CMS line (ICMA 93333A). Our method is especially important for developing inexpensive and high-throughput non-invasive genetic analyses.
Starting Page	114
Ending Page	122
Page Count	9
File Format	PDF
ISSN	12269239
Journal	Journal of Plant Biology
Volume Number	55
Issue Number	2
e-ISSN	18670725
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-11-09
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Direct-PCR DNA extraction Multiplex PCR Plant Breeding/Biotechnology Plant Genetics & Genomics Plant Ecology Plant Systematics/Taxonomy/Biogeography Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Plant Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Development of PCR-based diagnostics for soil borne plant pathogens

Genomic science for 21st century

Molecular cytogenetics of plant genome

MRNA Degradation Machinery in Plants

Analysis of gene expression upon DNA damage inArabidopsis

T-DNA integration patterns in transgenic tobacco plants

Germin-like proteins: structure, phylogeny, and function

Biogeography of North Pacific Isoëtes (Isoëtaceae) inferred from nuclear and chloroplast DNA sequence data

A gaseous plant hormone ethylene: the signaling pathway

A Simple and Non-destructive Method of Direct-PCR for Plant Systems

Similar Documents

Development of PCR-based diagnostics for soil borne plant pathogens

Genomic science for 21st century

Molecular cytogenetics of plant genome

MRNA Degradation Machinery in Plants

Analysis of gene expression upon DNA damage inArabidopsis

T-DNA integration patterns in transgenic tobacco plants

Germin-like proteins: structure, phylogeny, and function

Biogeography of North Pacific Isoëtes (Isoëtaceae) inferred from nuclear and chloroplast DNA sequence data

A gaseous plant hormone ethylene: the signaling pathway

A Simple and Non-destructive Method of Direct-PCR for Plant Systems