NDLI: Platycladus orientalis PoKub3 is involved in abiotic stress responses in transgenic arabidopsis

Content Provider	Springer Nature Link
Author	Yao, Xiamei Zhang, Jin Ji, Jing Yue, Jianyun Xie, Tiantian Deng, Nan Liu, Jianfeng Shi, Shengqing Jiang, Zeping Chang, Ermei
Copyright Year	2017
Abstract	Ku70-binding proteins associate with Ku70 and their expression levels can affect DSB repair efficiency via the DNA-PK-dependent repair pathway. However, how Ku70-binding proteins in plants exert a regulatory function under abiotic stress is poorly understood. Here, we cloned and characterized a PoKub3 gene from 500-year-old Platycladus orientalis. With increasing age, PoKub3 expression in P. orientalis increased gradually. The PoKub3 expression levels in leaves were upregulated under salt, heat, UV-C and abscisic acid treatments according to qRT-PCR. Moreover, PoKub3 overexpression in Arabidopsis thaliana improved tolerance to salt and drought stress compared with wild-type (WT) and vector control (VC) plants. High RAB18 and DREB2A expression and low JAZ1 and ABI2 expression provided strong evidence that salt tolerance was enhanced in the overexpression plants. Similarly, high RAB18 and DREB2A expression, accompanied by low JAZ1 and LOX1 expression and high DREB1A, CPK10, GSTF6 and APX1 expression, suggested the drought tolerance mechanism was associated with the abscisic acid pathway. In addition, lower malondialdehyde content, electrolyte leakage and stomatal conductance, and higher soluble sugar and relative water contents in PoKub3 overexpression lines than in WT and VC plants demonstrated its role in salt and drought tolerance. Together, these findings show that PoKub3 positively regulates salt and drought tolerance by regulating stress-related genes.
Starting Page	322
Ending Page	334
Page Count	13
File Format	PDF
ISSN	12269239
Journal	Journal of Plant Biology
Volume Number	60
Issue Number	4
e-ISSN	18670725
Language	English
Publisher	Springer Berlin Heidelberg
Publisher Date	2017-08-08
Publisher Place	Berlin, Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Abiotic stress Platycladus orientalis PoKub3 Transgenic Arabidopsis Plant Sciences Plant Breeding/Biotechnology Plant Genetics and Genomics Plant Systematics/Taxonomy/Biogeography Plant Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

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