NDLI: Characterization of PPO (polyphenol oxidase) cDNA in sweet persimmon (Diospyros kaki)

Content Provider	Springer Nature Link
Author	Bahn, Sung Chul Chung, Young Soo Shin, Jeong Sheop Lee, Chang Hoo Hyung, Nam In
Copyright Year	1999
Abstract	Polyphenol oxidases (PPOs) are copper metalloproteins which play a critical role in producing various derivatives of polyphenolic compounds. These enzymes are encoded in the nuclear genome and subsequently transported into the plastid. A partial cDNA clone encoding polyphenol oxidase was isolated from immature sweet persimmon leaves. Using two degenerate primers, a single band of PCR product was produced by the touchdown PCR method. In sequence analysis, this clone (define SPO; 1381 bp) revealed high similarities to the previously reported plant PPO genes. Moreover, this SPO clone was comprised of copper A and copper B-binding sites and a histidine-rich region, indicating that this clone contained the key conserved elements of PPO. To characterize the spatial and temporal expression of PPO, total RNAs were extracted from leaf and fruit peel tissues at different developmental stages. In northern analysis, transcripts of PPO were detected in all the samples tested, but the strongest expression levels were detected in mature fruit peels and leaves. Expression of PPO was inhibited in the transitional stage from chloroplast to chromoplast. In addition, the expression of PPO was highly induced in wounded immature fruit peel tissues.
Starting Page	16
Ending Page	22
Page Count	7
File Format	PDF
ISSN	12269239
Journal	Journal of Plant Biology
Volume Number	42
Issue Number	1
e-ISSN	18670725
Language	English
Publisher	Springer-Verlag
Publisher Date	1999-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Copper metalloprotein Degenerate primer PPO (Polyphenol Oxidase) Sweet Persimmon (Diospyros kaki) Touchdown PCR Plant Sciences Plant Breeding/Biotechnology Plant Genetics & Genomics Plant Systematics/Taxonomy/Biogeography Plant Ecology
Content Type	Text
Resource Type	Article
Subject	Plant Science

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