NDLI: Enhanced in vitro shoot multiplication and elongation in sugarcane using cefotaxime

Content Provider	Springer Nature Link
Author	Kaur, Ajinder Gill, M. S. Ruma, D. Gosal, S. S.
Copyright Year	2008
Abstract	Antibiotic cefotaxime has been found to be a growth promoting substance in sugarcane tissue culture. Young leaf segments (1–2 cm long) of three commercially important Indian sugarcane varieties viz., CoJ 83, CoJ 85 and Co 89003, regenerated into shoots and multiplied on semi-solid MS medium supplemented with 6-benzylaminopurine (BA, 0.2 mg/l) and kinetin (0.2 mg/l) for 2 cycles of 2 weeks each. Shoots (2 cm long) were separated and cultured on semi-solid half-strength MS medium supplemented with NAA (2 mg/l) and cefotaxime at various concentrations viz., 0, 250, 500 and 750 mg/l. Maximum shoot multiplication and elongation with respect to number of microtillers, shoot length and plantlet fresh weight in all the genotypes was obtained with cefotaxime used at the rate of 250 and 500 mg/l in the medium. Among the different varieties, on the basis of mean of 50 shoot cultures, number of microtillers per culture was highest in Co 89003 (7.50, 145.90% increase over control), whereas, shoot length and plantlet fresh weight were highest in CoJ 83, i.e. 10.30 cm (60.94% increase over control) and 360.45 mg (50.75% increase over control), respectively after 2 weeks of culturing with cephotaxime used at the rate of 500 mg/l in the medium. Statistical analysis of data revealed significant differences among varieties and media for different shoot multiplication and elongation parameters. Therefore, use of cefotaxime during tissue culture and genetic transformation of sugarcane can improve frequency of shoot multiplication and transformation, respectively.
Starting Page	60
Ending Page	64
Page Count	5
File Format	PDF
ISSN	09721525
Journal	Sugar Tech
Volume Number	10
Issue Number	1
e-ISSN	09740740
Language	English
Publisher	Springer-Verlag
Publisher Date	2008-06-01
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Young leaf segments tissue culture microtillers shoot length fresh weight antibiotic Agriculture
Content Type	Text
Resource Type	Article
Subject	Agronomy and Crop Science

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