NDLI: Assessment of Genetic Diversity in Sugarcane Germplasm Using ISSR and SSR Markers

Content Provider	Springer Nature Link
Author	Devarumath, Rachayya M. Kalwade, Sachin B. Kawar, Prashant G. Sushir, Kapil V.
Copyright Year	2012
Abstract	Molecular diversity within and between Saccharum species clones and elite commercial hybrid varieties was studied using intersimple sequence repeat (ISSR) and simple sequence repeat (SSR) markers. The present study was performed to characterize 81 sugarcane genotypes. A total of 13 ISSR primers used and produced 65 amplified fragments, of which 63 (96.5 %) were polymorphic. The Polymorphic Information Content (PIC) value ranged from 0.11 (UBC824) to 0.45 (UBC825) primers with an average value of 0.28. The primer UBC 817 and UBC 825 exhibited highest resolving power (Rp) value 3.8 among thirteen primers. Genetic similarity (GS) by Jaccard’s similarity coefficient ranged from 0.23 to 0.95 with a mean of 0.59. Of the 79 alleles amplified by 28 SSRs primers showed 76 alleles, which were found to be polymorphic (96.2 %). The PIC value ranged from 0.06 (VSICRAD4) to 0.55 (VSICRAD26) primers with an average value of 0.17. The primer VSICRAD23 exhibited highest resolving power (Rp) value 4.3 among 28 primers. The GS by Jaccard’s similarity coefficient ranged from 0.11 to 0.91 with a mean of 0.51. Dendrograms constructed using the UPGMA cluster analysis revealed low level of correlation between genetic similarities based on pedigree and DNA profiles. The ISSR and SSR amplification proved to be valuable method for assessing genetic diversity among sugarcane complex and their related wild varieties and for identification of the cultivars.
Starting Page	334
Ending Page	344
Page Count	11
File Format	PDF
ISSN	09721525
Journal	Sugar Tech
Volume Number	14
Issue Number	4
e-ISSN	09740740
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-07-04
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Genetic diversity Sugarcane ISSR SSR markers Saccharum Agriculture
Content Type	Text
Resource Type	Article
Subject	Agronomy and Crop Science

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Genetic Diversity of Populations of Saccharum spontaneum with Different Ploidy Levels Using SSR Molecular Markers

Analysis of Genetic Diversity of Saccharum Complex Using Chloroplast Microsatellite Markers

Genetic Diversity Among Saccharum spontaneum Clones and Commercial Hybrids Through SSR Markers

Genetic Diversity Analysis of Sugarcane Germplasm Based on Fluorescence-Labeled Simple Sequence Repeat Markers and a Capillary Electrophoresis-based Genotyping Platform

Inter simple sequence repeat profile as a genetic marker system in sugarcane

New Polymorphic EST-SSR Markers in Sugarcane

SSR marker-based analysis of genetic relatedness among sugarcane cultivars (Saccharum spp. hybrids) from breeding programs in China and other countries

Highly Polymorphic Microsatellite DNA Markers for Sugarcane Germplasm Evaluation and Variety Identity Testing

Assessment of genetic diversity within and among Basmati and non-Basmati rice varieties using AFLP, ISSR and SSR markers

Assessment of Genetic Diversity in Sugarcane Germplasm Using ISSR and SSR Markers

Similar Documents

Genetic Diversity of Populations of Saccharum spontaneum with Different Ploidy Levels Using SSR Molecular Markers

Analysis of Genetic Diversity of Saccharum Complex Using Chloroplast Microsatellite Markers

Genetic Diversity Among Saccharum spontaneum Clones and Commercial Hybrids Through SSR Markers

Genetic Diversity Analysis of Sugarcane Germplasm Based on Fluorescence-Labeled Simple Sequence Repeat Markers and a Capillary Electrophoresis-based Genotyping Platform

Inter simple sequence repeat profile as a genetic marker system in sugarcane

New Polymorphic EST-SSR Markers in Sugarcane

SSR marker-based analysis of genetic relatedness among sugarcane cultivars (Saccharum spp. hybrids) from breeding programs in China and other countries

Highly Polymorphic Microsatellite DNA Markers for Sugarcane Germplasm Evaluation and Variety Identity Testing

Assessment of genetic diversity within and among Basmati and non-Basmati rice varieties using AFLP, ISSR and SSR markers

Assessment of Genetic Diversity in Sugarcane Germplasm Using ISSR and SSR Markers