NDLI: Effect of Activated Charcoal, Carbon Sources and Gelling Agents on Direct Somatic Embryogenesis and Regeneration in Sugarcane via Leaf Roll Segments

Content Provider	Springer Nature Link
Author	Manchanda, Pooja Gosal, S. S.
Copyright Year	2012
Abstract	Efficient plant regeneration is an essential pre-requisite for a complete genetic transformation protocol. Direct somatic embryogenic cultures were established in commercial sugarcane varieties viz. CoJ 83 and CoH 119. Problem of browning of media was observed mainly in cultures of CoJ 83 as compared to CoH119. To study the effect of adsorption of phenolics by activated charcoal, two treatments were assayed in cv. CoJ 83 i.e. on Murashige and Skoog (MS) medium supplemented with α-Naphthalene acetic acid (NAA; 5 mgl$^{−1}$) and Kinetin (Kin; 0.5 mgl$^{−1}$) (control) and MS + NAA (5 mgl$^{−1}$) + Kin (0.5 mgl$^{−1}$) + charcoal (0.2% w/v) medium (treatment). In addition to control of browning of medium by phenolics, per cent somatic embryogenesis (SE) increased from 80.21 to 84.88 and per cent regeneration was also enhanced from 75.15 to 81.22 in sugarcane variety CoJ 83, when the medium was supplemented with 2,000 mgl$^{−1}$ of activated charcoal (AC). Further, the effect of addition of different carbon sources i.e. sucrose and maltose in four different concentrations 20, 25, 30 and 35 gl$^{−1}$ was studied and maximum per cent SE (98.35% in CoJ 83 and 93.12% in CoH 119; respectively) and maximum per cent regeneration (94.17% in CoJ 83 and 89.67% in CoH 119; respectively) was observed on MS + NAA (5 mgl$^{−1}$) and Kin (0.5 mgl$^{−1}$) medium supplemented with 25 gl$^{−1}$ maltose. Direct SE and regeneration was also affected considerably by varying the gelling agent (agar or gelrite) and its concentration. Best results were observed with increases in per cent SE and regeneration on medium solidified with agar (1.2%) compared to gelrite, when used as a gelling agent. Thus, this fairly simple activated charcoal (2% w/v), maltose (25 gl$^{−1}$) and agar (1.2%) containing direct SE medium provided an alternative method for improving SE and hence, regeneration frequency of sugarcane.
Starting Page	168
Ending Page	173
Page Count	6
File Format	PDF
ISSN	09721525
Journal	Sugar Tech
Volume Number	14
Issue Number	2
e-ISSN	09740740
Language	English
Publisher	Springer-Verlag
Publisher Date	2012-04-04
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Saccharum spp. Somatic embryogenesis Activated charcoal Regeneration Carbon sources Gelling agent Agriculture
Content Type	Text
Resource Type	Article
Subject	Agronomy and Crop Science

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