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Contribution of Ltype channels to Ca$^{2+}$ regulation of neuronal properties in early developing Purkinje neurons

Content Provider	Springer Nature Link
Author	Gruol, D. L. Netzeband, J. G. Quina, L. A. Blakely Gonzalez, P. K.
Copyright Year	2005
Abstract	Activity driven Ca$^{2+}$ signaling is an important regulator of neuronal development. Early developing Purkinje neurons (postnatal day 5–7) prior to the stage of dendritic development express a somatic Ca$^{2+}$ signaling pathway that is electrically driven and communicates information from the cell membrane to the cytosol and nucleus. In the current studies, we examined the properties and potential functional role of this pathway using acutely isolated Purkinje neurons from postnatal day 5–7 rat pups and brief K$^{+}$ stimulation to activate the pathway. Results show that the amplitude of the nuclear Ca$^{2+}$ 1signal increases as a function of the cytosolic Ca$^{2+}$ signal but is larger than the cytosolic Ca$^{2+}$ signal at strong K$^{+}$ stimulations. Both L-type and P-type Ca$^{2+}$ channels contribute to the Ca$^{2+}$ signal. We also show using semiquantitative immunohistochemical methods that activation of this Ca$^{2+}$ signaling pathway results in activation the transcription factor CREB and that L-type Ca$^{2+}$ channels play a prominent role in this effect. The level of cfos, a transcription factor whose expression is regulated by CREB, was also increased by K$^{+}$ stimulation. K$^{+}$ stimulation also altered the level of the Ca$^{2+}$ binding protein calbindin, an effect that involved L-type Ca$^{2+}$ channels. The relationship between increases in Ca$^{2+}$ and calbindin expression was bell-shaped, with high levels of Ca$^{2+}$ decreasing calbindin expression. The level of the transmitter GABA was also increased by K$^{+}$ stimulation but this effect was not dependent on L-type Ca$^{2+}$ channels. Taken together, these results support a role for L-type channels in the phenotypic expression of Purkinje neuron properties during early development and suggest that the different activity patterns of early developing Purkinje neurons could be one mechanism for signaling the induction of specific genes through differences in cytosolic or nuclear Ca$^{2+}$.
Starting Page	128
Ending Page	139
Page Count	12
File Format	PDF
ISSN	14734222
Journal	The Cerebellum
Volume Number	4
Issue Number	2
e-ISSN	14734230
Language	English
Publisher	Springer-Verlag
Publisher Date	2005-01-01
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Ca$^{2+}$ signaling neuronal development nuclear Ca$^{2+}$ CICR CREB Neurosciences Neurobiology Neurology
Content Type	Text
Resource Type	Article
Subject	Neurology Neurology (clinical)

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