NDLI: An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

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An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

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An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

Content Provider	Springer Nature Link
Author	Choudhary, Sumitra Kumari Patel, Ashok Kumar Shekhawat, Smita Shekhawat, Narpat S.
Copyright Year	2017
Abstract	Momordica dioica Roxb. ex Willd., is a perennial and dioecious (2n = 28) plant of family Cucurbitaceae. Conventional methods of propagation through seeds, stem cuttings and rhizomatous/tuberous roots are inadequate for its mass cultivation as a vegetable crop. This paper reports an improved and efficient micropropagation method for wild female M. dioica using nodal explants. Shoot amplification was achieved using subculturing of in vitro raised shoots on MS medium supplemented with various concentrations of 6-benzylaminopurine (BAP) alone or in combination with indole-3-acetic acid (IAA). The maximum number of shoots (45.30 ± 3.83) with an average length 6.52 ± 0.89 cm were differentiated on MS medium containing 0.5 mg L$^{−1}$ BAP, 0.1 mg L$^{−1}$ IAA and additives (50 mg L$^{−1}$ ascorbic acid, 25 mg L$^{−1}$ each of adenine sulphate, citric acid and l-arginine). The cloned shoots were rooted ex vitro. Each shoot treated with 250 mg L$^{−1}$ IBA for 5 min produced 12.3 ± 1.33 with a mean length 5.4 ± 0.73 cm. More than 85% (46 plants) of ex vitro rooted plantlets were successfully hardened in a greenhouse with normal growth characteristics. In order to evaluate the genetic stability of micropropagated plants, the two PCR-based techniques, Random Amplified Polymorphic DNA (RAPD) and Inter Simple Sequence Repeats (ISSR) were used. The amplification patterns of the micropropagated and mother plant were monomorphic thus depicting genetic stability of the micropropagation system. This protocol could be effectively employed for the mass multiplication of wild female M. dioica, a popular summer vegetable crop.
Starting Page	713
Ending Page	722
Page Count	10
File Format	PDF
ISSN	09715894
Journal	Physiology and Molecular Biology of Plants
Volume Number	23
Issue Number	3
e-ISSN	09740430
Language	English
Publisher	Springer India
Publisher Date	2017-04-28
Publisher Place	New Delhi
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Cucurbitaceae Ex vitro rooting Genetic homogeneity Micropropagation Spine gourd Plant Sciences Plant Physiology Biological and Medical Physics, Biophysics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Molecular Biology

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An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop.

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An improved micropropagation system, ex vitro rooting and validation of genetic homogeneity in wild female Momordica dioica: an underutilized nutraceutical vegetable crop

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