NDLI: Biolistic transformation of Scoparia dulcis L.

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Biolistic transformation of Scoparia dulcis L.

Content Provider	Springer Nature Link
Author	Srinivas, Kota Muralikrishna, Narra Kumar, Kalva Bharath Raghu, Ellendula Mahender, Aileni Kiranmayee, Kasula Yashodahara, Velivela Sadanandam, Abbagani
Copyright Year	2016
Abstract	Here, we report for the first time, the optimized conditions for microprojectile bombardment-mediated genetic transformation in Vassourinha (Scoparia dulcis L.), a Plantaginaceae medicinal plant species. Transformation was achieved by bombardment of axenic leaf segments with Binary vector pBI121 harbouring β-glucuronidase gene (GUS) as a reporter and neomycin phosphotransferase II gene (npt II) as a selectable marker. The influence of physical parameters viz., acceleration pressure, flight distance, gap width & macroprojectile travel distance of particle gun on frequency of transient GUS and stable (survival of putative transformants) expressions have been investigated. Biolistic delivery of the pBI121 yielded the best (80.0 %) transient expression of GUS gene bombarded at a flight distance of 6 cm and rupture disc pressure/acceleration pressure of 650 psi. Highest stable expression of 52.0 % was noticed in putative transformants on RMBI-K medium. Integration of GUS and npt II genes in the nuclear genome was confirmed through primer specific PCR. DNA blot analysis showed more than one transgene copy in the transformed plantlet genomes. The present study may be used for metabolic engineering and production of biopharmaceuticals by transplastomic technology in this valuable medicinal plant.
Starting Page	61
Ending Page	68
Page Count	8
File Format	PDF
ISSN	09715894
Journal	Physiology and Molecular Biology of Plants
Volume Number	22
Issue Number	1
e-ISSN	09740430
Language	English
Publisher	Springer India
Publisher Date	2016-01-20
Publisher Place	New Delhi
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Scoparia dulcis L. Biolistic transformation GUS assay Axenic leaf explants Stable expression Plant Sciences Plant Physiology Biophysics and Biological Physics Cell Biology
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Molecular Biology

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