NDLI: Isolation and Expression analysis of OsPME1, encoding for a putative Pectin Methyl Esterase from Oryza sativa (subsp. indica)

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Erratum

Physiology and Molecular Biology of Plants : Volume 15, Issue 1, January 2009

Physiology and Molecular Biology of Plants : Volume 14

Isolation and Expression analysis of OsPME1, encoding for a putative Pectin Methyl Esterase from Oryza sativa (subsp. indica)

Content Provider	Springer Nature Link
Author	Kanneganti, Vydehi Gupta, Aditya Kumar
Copyright Year	2009
Abstract	Pectin Methyl Esterases (PMEs) play an essential role during plant development by affecting the mechanical properties of the plant cell walls. Recent studies indicated that PMEs play important role in pollen tube development. In this study, we isolated a 1.3 kb cDNA clone from rice panicle cDNA library. It contained a 1038 bp of open reading frame (ORF) encoding for a putative pectin methyl esterase of 345 aminoacids with a 20 aminoacid signal peptide and was hence designated as OsPME1 (Oryza s ativa Pectin Methyl Esterase 1). It contained the structural arrangement GXYXE and GXXDFIF, found in the active groups of all PMEs. OsPME1 gene product shared varying identities, ranging from 52 % to 33 % with PMEs from other plant species belonging to Brassicaceae, Fabaceae, Amaranthaceae and Funariaceae. Southern blot analysis indicated that PME1 exists as a single copy in the rice genome. Expression pattern analysis revealed that OsPME1 is expressed only in pollen grains, during the later stages of their development and was also regulated by various abiotic stress treatments and phytohormones. Functional characterization of this pollen specific PME from rice would enable us to understand its role in pollen development.
Starting Page	123
Ending Page	131
Page Count	9
File Format	PDF
ISSN	09715894
Journal	Physiology and Molecular Biology of Plants
Volume Number	15
Issue Number	2
e-ISSN	09740430
Language	English
Publisher	Springer-Verlag
Publisher Date	2009-06-28
Publisher Place	India
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Oryza sativa Pectin Methyl Esterase Gene Expression Cell wall and pollen development Cell Biology Biophysics/Biomedical Physics Plant Physiology Plant Sciences
Content Type	Text
Resource Type	Article
Subject	Physiology Plant Science Molecular Biology

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Isolation and Expression analysis of OsPME1, encoding for a putative Pectin Methyl Esterase from Oryza sativa (subsp. indica)

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Isolation and Expression analysis of OsPME1, encoding for a putative Pectin Methyl Esterase from Oryza sativa (subsp. indica)

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