NDLI: Molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii isolates in Shenyang, China

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Molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii isolates in Shenyang, China

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Journal of Microbiology : Volume 48, Issue 4, August 2010

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Journal of Microbiology : Volume 48, Issue 1, February 2010

Journal of Microbiology : Volume 47

Journal of Microbiology : Volume 46

Molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii isolates in Shenyang, China

Content Provider	Springer Nature Link
Author	Zhang, Jing Ping Zhu, Wan Tian, Su Fei Chu, Yun Zhuo Chen, Bai Yi
Copyright Year	2010
Abstract	The investigation was carried out to elucidate the molecular characteristics and resistant mechanisms of imipenem-resistant Acinetobacter baumannii. Thirty-seven isolates were collected from January 2007 to December 2007. The homology of the isolates was analyzed by both pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST). The genes of β-lactamases, adeB, and class 1 integron were polymerase chain reaction amplified. Genotype analysis of the 37 A. baumannii isolates by PFGE revealed the circulation of four PFGE types (A-D); the A- and B-type accounted for 48.6% and 40.5%, respectively. MLST showed the existence of three allelic profiles. The agar dilution method was carried out to determine the MIC of imipenem, in the absence or presence of carbonyl cyanide m-chlorophenylhydrazone (CCCP, 10 μg/ml). The MICs of the strains to imipenem were between 16 μg/ml and 128 μg/ml. When CCCP was added, a MIC decrease of at least four-fold was observed in 20 isolates, which belonged to the A- or C-type. AdeB and bla $_{PER-1}$ genes were each detected in 35 isolates, bla $_{OXA-23}$ gene in 34 isolates and bla $_{OXA-58}$-like gene in 24 isolates. All isolates harbored bla $_{OXA-51}$-like genes. No isolates carried the bla $_{IMP-1}$ gene. Integron was detected in 25 isolates, which mediated the resistance to aminoglycosides and rifampin. The epidemiologic data suggested that the increasing infection of A. baumannii in our hospital was mainly caused by the inter-hospital spread of two epidemic clones. The AdeABC efflux system may be the important factor that leads to the high level of imipenem-resistance in PFGE A-type.
Starting Page	689
Ending Page	694
Page Count	6
File Format	PDF
ISSN	12258873
Journal	Journal of Microbiology
Volume Number	48
Issue Number	5
e-ISSN	19763794
Language	English
Publisher	The Microbiological Society of Korea
Publisher Date	2010-11-03
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	A. baumannii PFGE MLST β-lactamase efflux system integron Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology Applied Microbiology and Biotechnology

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