NDLI: Significance of the balance between intracellular glutathione and polyethylene glycol for successful release of small interfering RNA from gold nanoparticles

Please wait, while we are loading the content...

Chemically exfoliated WS$_{2}$ nanosheets efficiently inhibit amyloid β-peptide aggregation and can be used for photothermal treatment of Alzheimer’s disease

Achieving large transport bandgaps in bilayer graphene

M@MIL-100(Fe) (M = Au, Pd, Pt) nanocomposites fabricated by a facile photodeposition process: Efficient visible-light photocatalysts for redox reactions in water

Nanostructure and mechanical properties of the osteocyte lacunar-canalicular network-associated bone matrix revealed by quantitative nanomechanical mapping

Bandgap-tunable lateral and vertical heterostructures based on monolayer Mo$_{1-x }$W$_{ x }$S$_{2}$ alloys

Hybrid electromagnetic–triboelectric nanogenerator for harvesting vibration energy

Significance of the balance between intracellular glutathione and polyethylene glycol for successful release of small interfering RNA from gold nanoparticles

Magnetic control: Switchable ultrahigh magnetic gradients at Fe$_{3}$O$_{4}$ nanoparticles to enhance solution-phase mass transport

Direct detection and measurement of wall shear stress using a filamentous bio-nanoparticle

Large-scale synthesis of single-source, thermally stable, and dual-emissive Mn-doped Zn–Cu–In–S nanocrystals for bright white light-emitting diodes

Wafer-scale arrayed p-n junctions based on few-layer epitaxial GaTe

Pt$_{ x }$Cu$_{ y }$ nanocrystals with hexa-pod morphology and their electrocatalytic performances towards oxygen reduction reaction

An efficient and surface-benign purification scheme for colloidal nanocrystals based on quantitative assessment

Three-dimensional porous superaerophobic nickel nanoflower electrodes for high-performance hydrazine oxidation

Coherent Mn$_{3}$O$_{4}$-carbon nanocomposites with enhanced energy-storage capacitance

Spherical nano-Sb@C composite as a high-rate and ultra-stable anode material for sodium-ion batteries

First-principles computational study of highly stable and active ternary PtCuNi nanocatalyst for oxygen reduction reaction

A single Au nanoparticle anchored inside the porous shell of periodic mesoporous organosilica hollow spheres

Group 14 element-based non-centrosymmetric quantum spin Hall insulators with large bulk gap

A flexible magnetoelectric field-effect transistor with magnetically responsive nanohybrid gate dielectric layer

Low-temperature solution process for preparing flexible transparent carbon nanotube film for use in flexible supercapacitors

Erratum to: Serum-induced degradation of 3D DNA box origami observed with high-speed atomic force microscopy

Nano Research : Volume 8, Issue 9, September 2015

Nano Research : Volume 8, Issue 8, August 2015

Nano Research : Volume 8, Issue 7, July 2015

Nano Research : Volume 8, Issue 6, June 2015

Nano Research : Volume 8, Issue 5, May 2015

Nano Research : Volume 8, Issue 4, April 2015

Nano Research : Volume 8, Issue 3, March 2015

Nano Research : Volume 8, Issue 2, February 2015

Nano Research : Volume 8, Issue 1, January 2015

Nano Research : Volume 7

Nano Research : Volume 6

Nano Research : Volume 5

Nano Research : Volume 4

Nano Research : Volume 3

Nano Research : Volume 2

Nano Research : Volume 1

Significance of the balance between intracellular glutathione and polyethylene glycol for successful release of small interfering RNA from gold nanoparticles

Content Provider	Springer Nature Link
Author	McCully, Mark Hernandez, Yulan Conde, João Baptista, Pedro V. Fuente, Jesus M. Hursthouse, Andrew Stirling, David Berry, Catherine C.
Copyright Year	2015
Abstract	The therapeutic promise of small interfering RNAs (siRNAs) for specific gene silencing is dependent on the successful delivery of functional siRNAs to the cytoplasm. Their conjugation to an established delivery platform, such as gold nanoparticles, offers tremendous potential for treating diseases and advancing our understanding of cellular processes. Their success or failure is dependent on both the uptake of the nanoparticles into the cells and subsequent intracellular release of the functional siRNA. In this study, utilizing gold nanoparticle siRNA-mediated delivery against C-MYC, we aimed to determine if we could achieve knockdown in a cancer cell line with low levels of intracellular glutathione, and determine the influence, if any, of polyethylene glycol (PEG) ligand density on knockdown, with a view to determining the optimal nanoparticle design to achieve C-MYC knockdown. We demonstrate that, regardless of the PEG density, knockdown in cells with relatively low glutathione levels can be achieved, as well as the possible effect of steric hindrance of PEG on the availability of the siRNA for cleavage in the intracellular environment. Gold nanoparticle uptake was demonstrated via transmission electron microscopy and mass spectroscopy, while knockdown was determined at the protein and physiological levels (cells in S-phase) by in-cell westerns and BrdU incorporation, respectively.
Starting Page	3281
Ending Page	3292
Page Count	12
File Format	PDF
ISSN	19980124
Journal	Nano Research
Volume Number	8
Issue Number	10
e-ISSN	19980000
Language	English
Publisher	Tsinghua University Press
Publisher Date	2015-08-28
Publisher Place	Beijing
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	gold nanoparticles PEG glutathione siRNA drug delivery Nanotechnology Materials Science Atomic/Molecular Structure and Spectra Condensed Matter Physics Biotechnology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Nanoscience and Nanotechnology Atomic and Molecular Physics, and Optics Condensed Matter Physics Electrical and Electronic Engineering

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

The significance of the balance between intracellular glutathione and polyethylene glycol ( PEG ) for successful siRNA release from gold nanoparticles

Biomimetic gold nanocomplexes for gene knockdown: Will gold deliver dividends for small interfering RNA nanomedicines?

Proton-resistant quantum dots: Stability in gastrointestinal fluids and implications for oral delivery of nanoparticle agents

A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles

Raman Scattering Enhancement of Peg Coated Gold Nanoparticles of Defined Size

A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles.

PEGylation of double-walled carbon nanotubes for increasing their solubility in water

Bioconjugated silica nanoparticles: Development and applications

Sorafenib delivery nanoplatform based on superparamagnetic iron oxide nanoparticles magnetically targets hepatocellular carcinoma

Significance of the balance between intracellular glutathione and polyethylene glycol for successful release of small interfering RNA from gold nanoparticles

Similar Documents

The significance of the balance between intracellular glutathione and polyethylene glycol ( PEG ) for successful siRNA release from gold nanoparticles

Biomimetic gold nanocomplexes for gene knockdown: Will gold deliver dividends for small interfering RNA nanomedicines?

Proton-resistant quantum dots: Stability in gastrointestinal fluids and implications for oral delivery of nanoparticle agents

A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles

Raman Scattering Enhancement of Peg Coated Gold Nanoparticles of Defined Size

A colorimetric method for the molecular weight determination of polyethylene glycol using gold nanoparticles.

PEGylation of double-walled carbon nanotubes for increasing their solubility in water

Bioconjugated silica nanoparticles: Development and applications

Sorafenib delivery nanoplatform based on superparamagnetic iron oxide nanoparticles magnetically targets hepatocellular carcinoma

Significance of the balance between intracellular glutathione and polyethylene glycol for successful release of small interfering RNA from gold nanoparticles