NDLI: Dose- and time-dependent α-synuclein aggregation induced by ferric iron in SK-N-SH cells

Content Provider	Springer Nature Link
Author	Li, Wen Jing Jiang, Hong Song, Ning Xie, Jun Xia
Copyright Year	2010
Abstract	Intracellular formation of Lewy body (LB) is one of the hallmarks of Parkinson’s disease. The main component of LB is aggregated α-synuclein, present in the substantia nigra where iron accumulation also occurs. The present study was aimed to study the relationship between iron and α-synuclein aggregation.SK-N-SH cells were treated with different concentrations of ferric iron for 24 h or 48 h. MTT assay was conducted to determine the cell viability. Thioflavine S staining was used to detect α-synuclein aggregation.With the increase of iron concentration, the cell viability decreased significantly. At the concentrations of 5 mmol/L and 10 mmol/L, iron induced α-synuclein aggregation more severely than at the concentration of 1 mmol/L. Besides, 48-h treatment-induced aggregation was more severe than that induced by 24-h treatment, at the corresponding iron concentrations.Ferric iron can induce α-synuclein aggregation, which is toxic to the cells, in a dose- and time-dependent way.帕金森氏病的一个重要标志是胞浆内路易小体的形成。路易小体的主要成分是α- 突触核蛋白的聚集体, 它和铁的积聚一同存在于黒质区。本实验旨在研究铁和α- 突触核蛋白聚集的关系。SK-N-SH 细胞在不同浓度的三价铁作用下, 分别孵育24 h 或48 h, 用MTT 法和硫磺素S 染色法分别检测细胞存活率、观察α- 突触核蛋白的聚集情况。随着铁离子浓度的升高, 细胞存活率显著下降。其中5 mmol/L 和10 mmol/L 三价铁引起的α- 突触核蛋白的聚集相较于1 mmol/L 的三价铁引起的聚集更为严重。此外, 在同一浓度的三价铁作用下, 孵育48 h引起的聚集比孵育24 h引起的聚集更严重。铁离子诱发的α-突触核蛋白的聚集具有剂量和时间的依赖性, 并且α- 突触核蛋白的聚集介导了铁的毒性作用。
Starting Page	205
Ending Page	210
Page Count	6
File Format	PDF
ISSN	16737067
Journal	Neuroscience Bulletin
Volume Number	26
Issue Number	3
e-ISSN	19958218
Language	Chinese
Publisher	Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Publisher Date	2010-06-03
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Parkinson’s disease α-synuclein ferric ammonium citrate Pain Medicine Neurology Anatomy Anesthesiology Human Physiology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Neuroscience Physiology Medicine

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