NDLI: Effect of nitric oxide on the expression of apelin receptor mRNA in rat caudate nucleus

Content Provider	Springer Nature Link
Author	Bai, Bo Liu, Yu Hong Liu, Hai Qing
Copyright Year	2007
Abstract	To investigate the effect of nitric oxide (NO) on the expression of apelin receptor mRNA, as well as their correlation, in the caudate nucleus of rat.L-Arginine (L-Arg), N $^{ G }$-nitro-L-arginine methyl ester (L-NAME) and normal saline (NS) was separately microinjected into rat caudate nucleus. Expressions of neuronal NO synthase (nNOS) mRNA and apelin receptor mRNA were detected by RT-PCR at 4, 8, 12, 24 and 48 h after microinjection, and their correlation was determined.The expressions of nNOS mRNA and apelin receptor mRNA were both significantly increased after microinjection of L-Arg, but significantly decreased after microinjection of L-NAME compared with the NS control group. The nNOS mRNA had a positive correlation with the expression of apelin receptor mRNA after microinjection of L-Arg and L-NAME.The activity of NOS in the central nervous system, especially in the caudate nucleus, is one of the key factors for NO to exert many kinds of biological actions, such as modulation of central pain, as a neurotransmitter. The neurobiological action of NO in rat caudate nucleus may be associated with apelin receptors.研究大鼠尾核内一氧化氮(nitric oxide, NO)的作用机理及其与apelin 受体mRNA 表达的相关性。大鼠尾核内微量注射NO前体L-精氨酸(L-Arg)、 N $^{ G }$-硝基-L-精氨酸甲酯(N $^{ G }$-nitro-L-arginine methyl ester, L-NAME)和生理盐水(NS), 应用逆转录-聚合酶链反应(RT-PCR)检测大鼠尾核给药4、 8、 12、 24 及 48 h 后一氧化氮合酶(neuronal nitric oxide synthase, nNOS) mRNA 和 apelin 受体 mRNA 表达的变化及二者的相关性。注射 L-Arg 后大鼠尾核nNOS和apelin受体的mRNA表达明显升高, 注射 L-NAME 后大鼠尾核nNOS和apelin 受体mRNA表达明显降低, 且均有统计学意义。注射L-Arg 和L-NAME 后, nNOSmRNA 和 apelin 受体 mRNA 的表达呈正相关。在中枢神经系统, 尤其是在尾核中, NOS的活性是 NO 作为神经递质或调质参与包括中枢痛觉调制作用在内的多种生物学作用的关键因素之一, 尾核内NO的神经生物学作用可能与apelin受体作用相关。
Starting Page	180
Ending Page	184
Page Count	5
File Format	PDF
ISSN	16737067
Journal	Neuroscience Bulletin
Volume Number	23
Issue Number	3
e-ISSN	19958218
Language	Chinese
Publisher	Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences
Publisher Date	2008-02-03
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	nitric oxide apelin receptor pain caudate nucleus reverse transcription polymerase chain reaction Pain Medicine Neurology Anatomy Anesthesiology Human Physiology Neurosciences
Content Type	Text
Resource Type	Article
Subject	Neuroscience Physiology Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Nitric oxide inhibits excitatory vagal afferent input to nucleus tractus solitarius neurons in anaesthetized rats

Identification of differentially expressed genes in dorsal root ganglion in early diabetic rats

Differential effects of dopamine on pain-related electric activities in normal rats and morphinistic rats

Facial pain induces the alteration of transient receptor potential vanilloid receptor 1 expression in rat trigeminal ganglion

Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats

Oral arginine improves linear growth of long bones and the neuroendocrine mechanism

Properties of tactile responses of neurons in rat thalamic ventroposterolateral nucleus

Research progress on neurobiology of neuronal nitric oxide synthase

Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green

Effect of nitric oxide on the expression of apelin receptor mRNA in rat caudate nucleus

Similar Documents

Nitric oxide inhibits excitatory vagal afferent input to nucleus tractus solitarius neurons in anaesthetized rats

Identification of differentially expressed genes in dorsal root ganglion in early diabetic rats

Differential effects of dopamine on pain-related electric activities in normal rats and morphinistic rats

Facial pain induces the alteration of transient receptor potential vanilloid receptor 1 expression in rat trigeminal ganglion

Down-regulation of APLP1 mRNA expression in hippocampus of pilocarpine-induced epileptic rats

Oral arginine improves linear growth of long bones and the neuroendocrine mechanism

Properties of tactile responses of neurons in rat thalamic ventroposterolateral nucleus

Research progress on neurobiology of neuronal nitric oxide synthase

Rat pro-inflammatory cytokine and cytokine related mRNA quantification by real-time polymerase chain reaction using SYBR green

Effect of nitric oxide on the expression of apelin receptor mRNA in rat caudate nucleus