NDLI: Effects of environmental conditions and methanol feeding strategy on lipase-mediated biodiesel production using soybean oil

Content Provider	Springer Nature Link
Author	Jung, Sang Min Park, Yong Cheol Park, Kyungmoon
Copyright Year	2010
Abstract	Methanol is a commonly used acyl acceptor for lipase-driven biodiesel production, but a high concentration of methanol is detrimental for lipase activity. To overcome this drawback, a simple fed-batch process was developed by optimization of the methanol feeding strategy and reaction conditions. For the feeding strategy, an equal volume of pure methanol was fed twice with specified time intervals into a reactor initially containing a 1:1 molar ratio of soybean oil to methanol in order to adjust the net molar ratio of the oil to methanol to 1:3. In contrast with the batch reaction, a higher agitation speed in the fed-batch process elevated the conversion yield of soybean oil to biodiesel. An agitation speed of 600 rpm and a reaction temperature of 70°C were chosen as the optimal environmental conditions. Residual lipase activities for the fed-batch operation at 40 ∼ 70°C and 600 rpm were 7.1 ± 1.4 times higher than that of the batch method at 40°C with the same agitation speed, indicating that methanol feeding can prevent significant deactivation of lipase. Finally, two times feeding methanol at 2 and 6 hr resulted in a biodiesel productivity of 10.7%/h and 94.9% final conversion yield under the optimal conditions.
Starting Page	614
Ending Page	619
Page Count	6
File Format	PDF
ISSN	12268372
Journal	Biotechnology and Bioprocess Engineering
Volume Number	15
Issue Number	4
e-ISSN	19763816
Language	English
Publisher	The Korean Society for Biotechnology and Bioengineering
Publisher Date	2010-09-09
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	biodiesel lipase soybean oil fed-batch methanol feeding agitation speed temperature Industrial and Production Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil

Lipase catalyzed transesterification of soybean oil using ethyl acetate, an alternative acyl acceptor

Enhanced acetoin production by Serratia marcescens H32 using statistical optimization and a two-stage agitation speed control strategy

A new methanol-feeding strategy for the improved production of β-galactosidase in high cell-density fed-batch cultures of Pichia pastoris Mut$^{+}$ strains

Combined phospholipase and lipase catalysis for biodiesel production from phospholipids-containing oil

A pH shift feeding strategy for increased enduracidin production during fed–batch fermentation by a deep–sea, bacterium, Streptomyces sp. MC079

Effects of temperature shift strategies on human preproinsulin production in the fed-batch fermentation of recombinantEscherichia coli

An effective acid catalyst for biodiesel production from impure raw feedstocks

Sonication-assisted production of biodiesel using soybean oil and supercritical methanol

Effects of environmental conditions and methanol feeding strategy on lipase-mediated biodiesel production using soybean oil

Similar Documents

Biodiesel production by enzymatic process using Jatropha oil and waste soybean oil

Lipase catalyzed transesterification of soybean oil using ethyl acetate, an alternative acyl acceptor

Enhanced acetoin production by Serratia marcescens H32 using statistical optimization and a two-stage agitation speed control strategy

A new methanol-feeding strategy for the improved production of β-galactosidase in high cell-density fed-batch cultures of Pichia pastoris Mut$^{+}$ strains

Combined phospholipase and lipase catalysis for biodiesel production from phospholipids-containing oil

A pH shift feeding strategy for increased enduracidin production during fed–batch fermentation by a deep–sea, bacterium, Streptomyces sp. MC079

Effects of temperature shift strategies on human preproinsulin production in the fed-batch fermentation of recombinantEscherichia coli

An effective acid catalyst for biodiesel production from impure raw feedstocks

Sonication-assisted production of biodiesel using soybean oil and supercritical methanol

Effects of environmental conditions and methanol feeding strategy on lipase-mediated biodiesel production using soybean oil