NDLI: Production of milk clotting protease by a local isolate of Mucor circinelloides under SSF using agro-industrial wastes

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Production of milk clotting protease by a local isolate of Mucor circinelloides under SSF using agro-industrial wastes

Content Provider	Springer Nature Link
Author	Sathya, R. Pradeep, B. V. Angayarkanni, J. Palaniswamy, M.
Copyright Year	2009
Abstract	Agro-industrial residues, a cheap source of energy have high potential in the area of fermentation for the production of enzymes. Twenty agro-industrial residues were evaluated to check the possibility of potential utilization of substrates in SSF for milk clotting enzyme protease production by Mucor circinelloides. In this study, dhal husk holds the greatest promise for cost effective production of the milk clotting enzyme. The dhal husk supported maximum milk clotting protease production, and yield was improved with the supplementation of sucrose and yeast extract as carbon and nitrogen source, respectively. Among all the physico-chemical parameters tested, the best results were obtained in a medium having moisture content of 20% at pH 7.0, when inoculated with 30% of spore suspension and incubated at 30°C for 5 days. The activity was increased further on addition of Ca$^{2+}$, Cu$^{2+}$, and Mg$^{2+}$ ions. The purified milk-clotting protease obtained from M. circinelloides was successfully applied and compared with commercial rennet in the manufacture of a cheddar cheese.
Starting Page	788
Ending Page	794
Page Count	7
File Format	PDF
ISSN	12268372
Journal	Biotechnology and Bioprocess Engineering
Volume Number	14
Issue Number	6
e-ISSN	19763816
Language	English
Publisher	The Korean Society for Biotechnology and Bioengineering
Publisher Date	2010-01-14
Publisher Place	Heidelberg
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	dhal husk agro-industrial residues milk-clotting protease solid-state fermentation cheese production Industrial and Production Engineering Biotechnology
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Applied Microbiology and Biotechnology Biotechnology

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