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| Content Provider | Springer Nature Link |
|---|---|
| Author | Hong, Seung Pyo Lee, Chong Ho Kim, Se Kyung Yun, Hyun Shik Lee, Jung Heon Row, Kyung Ho |
| Copyright Year | 2004 |
| Abstract | Ceramide III was prepared by the cultivation ofSaccharomyces cerevisiae. Ceramide III was partitioned from the cell extracts by solvent extraction and analyzed by Normal Phase High Performance Liquid Chromatography (NP-HPLC) using Evaporative Light Scattering Detector (ELSD). We experimentally determined the mobile phase composition to separate ceramide III with NP-HPLC. Three binary mobile phases of n-hexane/ethanol,n-hexane/Isoprophyl Alcohol (IPA) andn-hexane/n-butanol and one ternary mobile phase ofn-hexane/IPA/methanol were demonstrated. For the binary mobile phase ofn-hexane/ethanol, the first mobile phase composition, 95/5 (v/v), was step-increased to 72/23 (v/v) at 3 min. In the binary mobile phase, the retention time of ceramide III was 7.87 min, while it was 4.11 min respectively in the ternary system, where the mobile phase composition ofn-hexane/IPA/methanol, 85/7/8 (v/v/v), was step-increased to 75/10/15 (v/v/v) at 3 min. However, in the ternary mobile phase, the more peak area of ceramide III was observed. |
| Starting Page | 47 |
| Ending Page | 51 |
| Page Count | 5 |
| File Format | |
| ISSN | 12268372 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume Number | 9 |
| Issue Number | 1 |
| e-ISSN | 19763816 |
| Language | English |
| Publisher | The Korean Society for Biotechnology and Bioengineering |
| Publisher Date | 2004-01-01 |
| Publisher Place | Seoul |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Saccharomyces cerevisiae cermaide III mobile phase NP-HPLC ELSD Biotechnology Industrial and Production Engineering |
| Content Type | Text |
| Resource Type | Article |
| Subject | Bioengineering Biomedical Engineering Applied Microbiology and Biotechnology Biotechnology |
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