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| Content Provider | Springer Nature Link |
|---|---|
| Author | Jung, Kyung Hwan Lee, You Jin Yeon, Ji Hyeon Yoo, Sun Kyun Chung, Byeong Churl |
| Copyright Year | 2009 |
| Abstract | To improve the soluble expression of recombinant human interferon-α that was directed by the araBAD promoter system of Escherichia coli, we attempted to control the overall protein expression rate via the addition of a repressor, methyl α-D-glucospyranoside (α-MG). Recombinant interferon-α was usually expressed as an inclusion body at the end of DO (dissolved oxygen)-stat fed-batch culture. However, the addition of 0.0025 to 0.01% α-MG after 0.5% L-arabinose induction effectively inhibited a tendency towards the formation of inclusion bodies, in which 67.6 to 73.1% of the expressed interferon-α was found in the soluble fraction. It was likely that the addition of a repressor after L-arabinose induction partially modulated the transcription rate from the araBAD promoter system and changed the ratio of soluble and insoluble interferon-α expression. This modulation might be considered as a method that can improve the soluble expression level of recombinant protein at the optimal temperature for cell growth. |
| Starting Page | 274 |
| Ending Page | 280 |
| Page Count | 7 |
| File Format | |
| ISSN | 12268372 |
| Journal | Biotechnology and Bioprocess Engineering |
| Volume Number | 14 |
| Issue Number | 3 |
| e-ISSN | 19763816 |
| Language | English |
| Publisher | The Korean Society for Biotechnology and Bioengineering |
| Publisher Date | 2009-07-14 |
| Publisher Place | Heidelberg |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | araBAD promoter methyl α-D-glucopyranoside soluble expression of interferon-α modulation of transcription Escherichia coli Industrial and Production Engineering Biotechnology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Bioengineering Biomedical Engineering Applied Microbiology and Biotechnology Biotechnology |
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