NDLI: Parameter estimation of perillyl alcohol in RP-HPLC by moment analysis

Content Provider	Springer Nature Link
Author	Row, Kyung Ho Lee, Chong Ho Kang, Ji Hoon
Copyright Year	2002
Abstract	Parameter estimations were made for the reversed-phase adsorption of perillyl alcohol (POH), a potent anti-cancer agent, on octadecylsilyl-silica gel (ODS). The average particle diameter of ODS was about 15 μm, and the particles were packed in the column (3.9 × 300 mm). The mobile phase used was a mixture of acetonitrile and water, in which the acetonitrile ranged between 50 and 70 (v/v %). The first absolute moment and the second central moment were determined from the chromatographic elution curves by moment analysis. Experiments were carried out using POH solutions within the linear adsorption range. The fluid-to-particle mass transfer coefficient was estimated using the Wilson-Geankoplis equation. The axial dispersion coefficient and the intraparticle diffusivity were determined from the slope and intercept of a plot ofH vs 1/u $_{0}$, respectively. The contributions of each mass-transfer step were axial dispersion, fluid-toparticle mass transfer, and intraparticle diffusion.
Starting Page	16
Ending Page	20
Page Count	5
File Format	PDF
ISSN	12268372
Journal	Biotechnology and Bioprocess Engineering
Volume Number	7
Issue Number	1
e-ISSN	19763816
Language	English
Publisher	The Korean Society for Biotechnology and Bioengineering
Publisher Date	2002-01-01
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	moment analysis parameter estimation perillyl alcohol Biotechnology Industrial and Production Engineering
Content Type	Text
Resource Type	Article
Subject	Bioengineering Biomedical Engineering Applied Microbiology and Biotechnology Biotechnology

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