NDLI: Protocol modifications influence the result of EGF receptor immunodetection by EGFR pharmDx$^{™}$ in paraffin-embedded cancer tissues

Content Provider	Springer Nature Link
Author	Derecskei, Katalin Moldvay, Judit Bogos, Krisztina Tímár, József
Copyright Year	2006
Abstract	EGF receptor (EGFR) became a useful target for several recently introduced therapies of various cancer types including colorectal, lung, head and neck cancers and glioblastoma. The successful clinical application of these novel molecularly targeted therapies requires the expression of their target, EGFR, determined by nucleic acid based or immunohistochemical techniques. However, until now, immunohistochemistry has not become a reliable diagnostic approach for this purpose. The golden standard for the determination of EGFR protein expression in paraffin-embedded cancer tissues is the EGFR pharmDx$^{™}$ kit. Here we show that the recommended protocol may not be optimal for EGFR immunodetection. Microwave antigen retrieval and extended primary antibody incubation time converted four out of eight EGFR-negative tumors into EGFR-positive in a study of 50 lung adenocarcinoma cases. Accordingly, we recommend retesting cases negative for EGFR with EGFR pharmDx$^{™}$ using protocol modifications optimizing antigen retrieval and the incubation periods.
Starting Page	243
Ending Page	246
Page Count	4
File Format	PDF
ISSN	12194956
Journal	Pathology & Oncology Research
Volume Number	12
Issue Number	4
e-ISSN	15322807
Language	English
Publisher	Springer Netherlands
Publisher Date	2006-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	EGF receptor immunodetection EGFR pharmDx$^{™}$ antigen retrieval incubation time Cancer Research Oncology Pathology Immunology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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