NDLI: In-Vitro and In-Vivo Imaging of Prostate Tumor Using NaYF$

Content Provider	Springer Nature Link
Author	Yu, Yongjiang Huang, Tao Wu, Yu Ma, Xiaorong Yu, Guopeng Qi, Jun
Copyright Year	2013
Abstract	The aim of this study was to investigate the feasibility of prostate tumor bioimaging both in vitro and in vivo using an upconversion fluorophore, NaYF$_{4}$: Yb, Er nanoparticles. Luminescent signals of human prostate cancer cells (CWR22R and LNCaP) labeled with NaYF$_{4}$: Yb, Er nanoparticles were detected by laser scanning confocal microscope, while Cy3 or FITC was used as control probe. Mouse-human prostate cancer model was developed by subcutaneously injecting the CWR22R cells into BALB/c nude mice to investigate the in-vivo imaging properties of NaYF$_{4}$:Yb, Er nanoparticles. Both CWR22R and LNCaP cells could phagocytose NaYF$_{4}$:Yb, Er nanoparticles in vitro, and the cellular uptake of CWR22R cells was much higher than that of LNCaP cells (95.42 ± 3.47 % vs. 51.63 ± 6.43 %), which made us choose the former for the further study. CWR22R cells pre-labeled with NaYF$_{4}$:Yb, Er nanoparticles showed no obvious decrease of fluorescence intensity (P > 0.05) after light exposure, while the fluorescence intensity of Cy3 or FITC labeled cells decreased rapidly with prolonged bleaching (P < 0.05). Furthermore, the in-vivo results showed that the prostate cancer cells pre-labeled with or without NaYF$_{4}$:Yb, Er nanoparticles formed tumors 4 weeks after injection, and the tumor length-diameter of the nanoparticle group and the control group was (10.3 ± 2.0) mm and (9.8 ± 2.5) mm, respectively. Significant upconversion fluorescence signals were observed in the tumors of the nanoparticle group when being excited at 980 nm by a NIR laser. In summary, the results suggest that as an intensive fluorescence imaging label agent, NaYF$_{4}$:Yb, Er nanoparticles possess unique features and can be used for imaging prostate tumor cells both in vitro and in vivo by phagocytosis.
Starting Page	335
Ending Page	341
Page Count	7
File Format	PDF
ISSN	12194956
Journal	Pathology & Oncology Research
Volume Number	20
Issue Number	2
e-ISSN	15322807
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-11-14
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	NaYF$_{4}$:Yb, Er Nanoparticles Prostate tumor cells Biological imaging Cancer Research Oncology Pathology Immunology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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