NDLI: Microsatellite analysis of chromosome 3p region in sporadic renal cell carcinomas

Content Provider	Springer Nature Link
Author	Girolami, Francesca Passerini, Ilaria Garga, Dorotea Frusconi, Sabrina Villari, Donata Nicita, Giulio Torricelli, Francesca
Copyright Year	2002
Abstract	The etiology and progression of renal carcinomas (RCC) is still poorly understood. RCC have been classified into several pathological entities. The most frequent type, clear cell carcinoma, accounts for about 80% of sporadic RCC and shows several chromosome abnormalities documented both by conventional cytogenetics, loss of eterozygosity (LOH) and replication error (RER) studies. In 10 clear cell type sporadic RCC we evaluated LOH and RER using a set of 10 micro satellite markers covering the chromosome 3p region, which has been suggested for interstitial deletions. Electrophoresis was performed by automated sequencer ABI Prism 377 and data were analyzed with Genescan and Genotyper 2.5 softwares. We revealed allelic loss in 48,7% of informative microsatellites and a single case of RER. We found the highest LOH frequency in 3p25-26 region where maps Von Hippel-Lindau (VHL) oncosuppressor gene. In addition, DNA hypermethylation, an alternative mechanism of VHL gene silencing, was evaluated by methylation-specific PCR. However hypermethylation status was not detected in any of our tumor samples.
Starting Page	241
Ending Page	244
Page Count	4
File Format	PDF
ISSN	12194956
Journal	Pathology & Oncology Research
Volume Number	8
Issue Number	4
Language	English
Publisher	Springer Netherlands
Publisher Date	2002-01-01
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	microsatellite instability renal cell carcinoma LOH RER Cancer Research Oncology Pathology Immunology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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