NDLI: High Frequency of Temperature-Sensitive Mutants of p53 in Glioblastoma

Content Provider	Springer Nature Link
Author	Smardova, Jana Liskova, Kvetoslava Ravcukova, Barbora Kubiczkova, Lenka Sevcikova, Sabina Michalek, Jaroslav Svitakova, Miluse Vybihal, Vaclav Kren, Leos Smarda, Jan
Copyright Year	2013
Abstract	Glioblastoma is the most common and the most aggressive type of brain cancer. Aberrations of the RTK/RAS/PI3K-, p53-, and RB cell signaling pathways were recognized as a core requirement for pathogenesis of glioblastoma. The p53 tumor suppressor functions as a transcription factor transactivating expression of its target genes in response to various stress stimuli. We determined the p53 status in 36 samples of glioblastoma by functional analyses FASAY and split assay. Seventeen p53 mutations were detected and further analyzed by cDNA and gDNA sequencing in 17 patients (47.2 %). Fifteen (88.2 %) of the mutations were missense mutations causing amino acid substitutions, seven of them exhibited temperature-sensitivity. Two mutations were determined as short deletions, one of them causing formation of premature termination codon in position 247. Fluorescent in situ hybridization revealed the loss of the p53-specific 17p13.3 locus in four of 33 analyzed samples (12 %). In 12 out of 30 samples (40 %), the p53 protein accumulation was shown by immunoblotting. There was high (80 %) concordance between the presence of the clonal p53 mutation and the p53 protein accumulation.
Starting Page	421
Ending Page	428
Page Count	8
File Format	PDF
ISSN	12194956
Journal	Pathology & Oncology Research
Volume Number	19
Issue Number	3
e-ISSN	15322807
Language	English
Publisher	Springer Netherlands
Publisher Date	2013-03-28
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Glioblastoma p53 tumor suppressor FASAY Temperature-sensitive mutations, p53 gene deletion Cancer Research Oncology Pathology Immunology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pathology and Forensic Medicine Oncology

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