NDLI: Expression of outer capsid protein VP5 of grass carp reovirus in E.coli and analysis of its immunogenicity

Content Provider	Springer Nature Link
Author	Zhang, Lan lan Shen, Jin yu Lei, Cheng feng Fan, Chao Hao, Gui jie Fang, Qin
Copyright Year	2009
Abstract	Grass carp reovirus (GCRV) is a tentative member of the Aquareovirus genus in the family Reoviridae. The mature virion comprises 11 dsRNA genomes enclosed by two concentric icosahedral proteins shells that is comprised of five core proteins and two outer capsid proteins. The genome sequence and 3D structure demonstrate there is a higher level of sequence homology in structural proteins between GCRV and mammalian orthoreoviruses (MRV) compared to other members of the family. To understand the pathogenesis of GCRV infection, the outer capsid protein VP5, a homology of the μ1 protein of MRV, was expressed in E.coli. It was found that the recombinant VP5 was highly expressed, and the expressed His-tag fusion protein was involved in the formation of the inclusion body. Additionally, specific anti-VP5 serum was prepared from purified protein and western blot demonstrated that the expressed protein was able to bind immunologically to rabbit anti GCRV particle serum and the immunogenicity was determined by ELISA assay. Additional experiments in investigating the functional properties of VP5 will further elucidate the role of the GCRV outer capsid protein VP5 during entry into host cells, and its interaction among viral proteins and host cells during the infection process.
Starting Page	545
Ending Page	551
Page Count	7
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	24
Issue Number	6
e-ISSN	1995820X
Language	English
Publisher	SP Wuhan Institute of Virology, CAS
Publisher Date	2009-11-27
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	Grass carp reovirus (GCRV) Outer capsid protein VP5 Expression in E.coli Immunogenicity Microbial Genetics and Genomics Microbiology Biochemistry Oncology Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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