NDLI: Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains

Content Provider	Springer Nature Link
Author	Saha, Dipongkor Karniychuk, Uladzimir U. Huang, Liping Geldhof, Marc Vanhee, Merijn Lefebvre, David J. Meerts, Peter Ducatelle, Richard Doorsselaere, Jan V. Nauwynck, Hans J.
Copyright Year	2014
Abstract	VC2002, isolated from postweaning multisystemic wasting syndrome (PMWS)-affected pig, is a mixture of two porcine circovirus genotype 2b (PCV2b) viruses, K2 and K39. Preliminary experiments disclosed short-term adverse effects of K39, but not K2, on porcine foetuses. These findings led to the hypothesis that infection of immuno-incompetent foetuses with K2 confers a status of immunotolerance, and postnatal super-infection with K39 triggers PMWS. To explore this hypothesis, nine 55-day-old foetuses were inoculated in utero (three with K2-10$^{4.3}$TCID$_{50}$, three with K39-10$^{4.3}$TCID$_{50}$ and three with medium), and foeto-pathogenicity examined. At 21 days post-inoculation (dpi), K2 did not induce pathology, whereas pathological effects of K39 were evident. Twenty-four 45-day-old foetuses were subsequently inoculated to examine the long-term effect of K2, including six with K2-high dose-10$^{4.3}$TCID$_{50}$, six with K2-low dose-10$^{2.3}$TCID$_{50}$ and 12 mock-inoculated controls. Both doses resulted in five mummified foetuses and one live-born piglet each (69dpi). K2 was recovered from all mummies. K2 and K2-specific antibodies were not detected in serum of the two live-born piglets at birth, indicating full control of K2 infection. The K2-low dose-infected piglet was immunostimulated at day 2, but not the K2-high dose-infected piglet. Both non-stimulated and stimulated K2-infected piglets were super-inoculated with K39 at day 6 or 8 (taken as 0 days post super-inoculation). Low viral replication was observed in the non-stimulated K2-K39 piglet (up to 10$^{3.3}$TCID$_{50}$/g; identified as K39). In contrast, viral replication was extremely high in the stimulated K2-K39 piglet (up to 10$^{5.6}$TCID$_{50}$/g) and identified as K2, indicating that K2 infection is controlled during foetal life, but emerges after birth upon immunostimulation. However, none of the piglets showed any signs of PMWS.
Starting Page	176
Ending Page	182
Page Count	7
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	29
Issue Number	3
e-ISSN	1995820X
Language	English
Publisher	Wuhan Institute of Virology, CAS
Publisher Date	2014-06-17
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	PCV2 immunotolerance PMWS porcine foetuses Virology Medical Microbiology Oncology Biochemistry Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains

Anti-porcine circovirus type 2 (PCV2) antibody placental barrier leakage from sow to fetus: impact on the diagnosis of intra-uterine PCV2 infection

Development of a loop-mediated isothermal amplification assay for porcine circovirus type 2

An ELISA based on a truncated soluble ORF2 protein for the detection of PCV2 antibodies in domestic pigs

Histopathology of Porcine kobuvirus in Chinese piglets

Identification and genotyping of porcine circovirus type II (PCV2) in Mexico.

Integrin activation and viral infection

Ultrastructural features of PPRV infection in Vero cells

Caspase work model during pathogen infection

Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains

Similar Documents

Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains

Anti-porcine circovirus type 2 (PCV2) antibody placental barrier leakage from sow to fetus: impact on the diagnosis of intra-uterine PCV2 infection

Development of a loop-mediated isothermal amplification assay for porcine circovirus type 2

An ELISA based on a truncated soluble ORF2 protein for the detection of PCV2 antibodies in domestic pigs

Histopathology of Porcine kobuvirus in Chinese piglets

Identification and genotyping of porcine circovirus type II (PCV2) in Mexico.

Integrin activation and viral infection

Ultrastructural features of PPRV infection in Vero cells

Caspase work model during pathogen infection

Unusual outcome of in utero infection and subsequent postnatal super-infection with different PCV2b strains