NDLI: HIV-1 Vif protein mediates the degradation of APOBEC3G in fission yeast when over-expressed using codon optimization

Content Provider	Springer Nature Link
Author	Li, Lin Li, Jing yun Sui, Hong shuai Zhao, Richard Y. Liu, Yong jian Bao, Zuo yi Liu, Si yang Zhuang, Dao min
Copyright Year	2008
Abstract	Interaction between the HIV-1 Vif protein and the cellular host APOBEC3G protein is a promising target for inhibition of HIV-1 replication. Considering that human cells are a very complicated environment for the study of protein interactions, the goal of this study was to check whether fission yeast could be used as a model cell for studying the Vif-APOBEC3G interaction. Vif and APOBEC3G were expressed in fusion with GFP protein in the S. pombe SP223 strain. Subcellular localizations of Vif and APOBEC3G were observed with fluorescent microscopy. Codon optimization was used to over express the Vif protein in S. pombe cells. The degradation of APOBEC3G mediated by Vif was tested through expressing Vif and GFP-APOBEC3G proteins in the same cell. Western Blot analysis was used to measure the corresponding protein levels under different experimental conditions. The results showed that the Vif protein was predominantly localized in the nucleus of S. pombe cells, APOBEC3G was localized in the cytoplasm and concentrated at punctate bodies that were often in close proximity to the nucleus but were not necessarily restricted from other regions in the cytoplasm. Vif protein expression levels were increased significantly by using codon optimization and APOBEC3G was degraded when Vif was over-expressed in the same S. pombe cells. These results indicate that fission yeast is a good model for studying the interaction between the Vif and APOBEC3G proteins.
Starting Page	255
Ending Page	264
Page Count	10
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	23
Issue Number	4
e-ISSN	1995820X
Language	English
Publisher	SP Wuhan Institute of Virology, CAS
Publisher Date	2008-08-08
Publisher Place	Heidelberg
Access Restriction	Subscribed
Subject Keyword	HIV Vif protein APOBEC3G Fission yeast (Schizosaccharomyces pombe) Microbial Genetics and Genomics Microbiology Biochemistry Oncology Medical Microbiology Virology
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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