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| Content Provider | Springer Nature Link |
|---|---|
| Author | Ji, Xiuling Zhang, Chunjing Fang, Yuan Zhang, Qi Lin, Lianbing Tang, Bing Wei, Yunlin |
| Copyright Year | 2015 |
| Abstract | As a unique ecological system with low temperature and low nutrient levels, glaciers are considered a “living fossil” for the research of evolution. In this work, a lytic cold-active bacteriophage designated VMY22 against Bacillus cereus MYB41-22 was isolated from Mingyong Glacier in China, and its characteristics were studied. Electron microscopy revealed that VMY22 has an icosahedral head (59.2 nm in length, 31.9 nm in width) and a tail (43.2 nm in length). Bacteriophage VMY22 was classified as a Podoviridae with an approximate genome size of 18 to 20 kb. A one-step growth curve revealed that the latent and the burst periods were 70 and 70 min, respectively, with an average burst size of 78 bacteriophage particles per infected cell. The pH and thermal stability of bacteriophage VMY22 were also investigated. The maximum stability of the bacteriophage was observed to be at pH 8.0 and it was comparatively stable at pH 5.0–9.0. As VMY22 is a cold-active bacteriophage with low production temperature, its characterization and the relationship between MYB41-22 and Bacillus cereus bacteriophage deserve further study. |
| Starting Page | 52 |
| Ending Page | 58 |
| Page Count | 7 |
| File Format | |
| ISSN | 16740769 |
| Journal | Virologica Sinica |
| Volume Number | 30 |
| Issue Number | 1 |
| e-ISSN | 1995820X |
| Language | English |
| Publisher | Wuhan Institute of Virology, CAS |
| Publisher Date | 2015-02-02 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Bacillus cereus characterization cold-active phage lytic Podoviridae Virology Medical Microbiology Oncology Biochemistry Microbiology Microbial Genetics and Genomics |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Virology Immunology Molecular Medicine |
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