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| Content Provider | Springer Nature Link |
|---|---|
| Author | Dong, Dan Fu, Shi hong Wang, Li hua Lv, Zhi Li, Tai yuan Liang, Guo dong |
| Copyright Year | 2012 |
| Abstract | Arboviruses represent a serious problem to public health and agriculture worldwide. Fast, accurate identification of the viral agents of arbovirus-associated disease is essential for epidemiological surveillance and laboratory investigation. We developed a cost-effective, rapid, and highly sensitive one-step “triplex RT-PCR enzyme hybridization” assay for simultaneous detections of Japanese Encephallitis virus (JEV, Flaviviridae), Getah virus (GETV, Togaviridae), and Tahyna virus (TAHV, Bunyaviridae) using three pairs of primers to amplify three target sequences in one RT-PCR reaction. The analytical sensitivity of this assay was 1 PFU/mL for JEV, 10 PFU/mL for GETV, and 10 PFU/mL for TAHV. This assay is significantly more rapid and less expensive than the traditional serological detection and single RT-PCR reaction methods. When “triplex RT-PCR enzyme hybridization” was applied to 29 cerebrospinal fluid (CSF) samples that were JEV-positive by normal RT-PCR assay, all samples were strongly positive for JEV, but negative for GETV and TAHV, demonstrating a good sensitivity, specificity, and performance at CSF specimen detection. |
| Starting Page | 179 |
| Ending Page | 186 |
| Page Count | 8 |
| File Format | |
| ISSN | 16740769 |
| Journal | Virologica Sinica |
| Volume Number | 27 |
| Issue Number | 3 |
| e-ISSN | 1995820X |
| Language | English |
| Publisher | SP Wuhan Institute of Virology, CAS |
| Publisher Date | 2012-06-09 |
| Publisher Place | Heidelberg |
| Access Restriction | Subscribed |
| Subject Keyword | Japanese Encephalitis virus (JEV) Getah Virus (GETV) Tahyna Virus (TAHV) Multiplex RT-PCR Enzyme Hybridization Microbial Genetics and Genomics Oncology Medical Microbiology Biochemistry Virology Microbiology |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Virology Immunology Molecular Medicine |
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