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Establishment, growth kinetics, and susceptibility to AcMNPV of heat tolerant lepidopteran cell lines

Content Provider	Springer Nature Link
Author	Wu, Yan lei Jiang, Lei Hashimoto, Yoshifumi Granados, Robert R. Li, Guo xun
Copyright Year	2011
Abstract	Lepidopteran heat-tolerant (ht) cell lines have been obtained with sf-9, sf-21 and several Bombyx cells. They have a distinct karyotype, membrane lipid composition, morphology and growth kinetics from the parental cell lines. In this paper, we report the development of ht cell lines from other insect species and examination of their growth characteristics and virus susceptibility. Adaptation of cell lines sf-9, BTI-TN-5B1-4 (High5) and BTI-TN-MG1 (MG1) to 33°C and 35°C was carried out by shifting the culture temperature between 28°C and higher temperatures by a gradual stepwise increase in temperature. The process of adaption to a higher culture temperature was accomplished over a period of 2 months. The cell lines with the temperature adaption were designated as sf9-ht33, sf9-ht35, High5-ht33, High5-ht35, MG1-ht33, MG1-ht35. These cell lines have been subcultured over 70 passages. Adaption to high temperatures was confirmed by a constant population doubling time with individual cell lines. The population doubling time of heat adapted cell lines were 1–4 h less than these of parental cell lines. Cell shapes did not show obvious change, however, the cell size of sf9-ht cells was enlarged and those of High5 and MG1 ht cells were reduced after heat adaption. When the cell lines were infected with Autographa californica nuclear polyhedrosis virus (AcMNPV) at 28°C, 33°C, 35°C and 37°C, production of budded virus and occlusion bodies in each cell line was optimum at its own adapted temperature.
Starting Page	198
Ending Page	205
Page Count	8
File Format	PDF
ISSN	16740769
Journal	Virologica Sinica
Volume Number	26
Issue Number	3
e-ISSN	1995820X
Language	English
Publisher	Springer-Verlag
Publisher Date	2011-06-12
Publisher Place	Berlin, Heidelberg
Access Restriction	Subscribed
Subject Keyword	Heat-tolerant cell lines Susceptibility of virus Production of virus Growth curves Insect cell lines Virology Medical Microbiology Oncology Biochemistry Microbiology Microbial Genetics and Genomics
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Virology Immunology Molecular Medicine

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