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| Content Provider | Springer Nature Link |
|---|---|
| Author | Wickramasinghe, W. M. D. K. S. Qu, Xinshun Costanzo, Stefa Haynes, Kathleen G. Christ, Barbara J. |
| Copyright Year | 2009 |
| Abstract | A diploid hybrid Solanum phureja x S. stenotomum population that has shown relatively high levels of quantitative resistance to late blight was used to develop polymerase chain reaction (PCR)-based markers for marker-assisted selection (MAS) of disease resistance in potato breeding. Two random amplified polymorphic DNA (RAPD) markers, OPA03$_{576}$ and OPA17$_{559}$, were identified as being linked to partial resistance. OPA03$_{576}$ was found to be associated in coupling phase while OPA17$_{559}$ showed association in repulsion phase to resistance. These two markers, together with previously developed amplified fragment length polymorphism (AFLP) and simple sequence repeat (SSR) markers, were incorporated into an existing restriction fragment length polymorphism (RFLP)-based chromosomal linkage map of this S. phureja x S. stenotomum population. Linkage analysis located the OPA17$_{559}$ marker on chromosome III, 12 cM away from a major quantitative trait locus (QTL) that explained 23.4% of the total phenotypic variation for late blight resistance. Marker OPA03$_{576}$ was located in the distal region of chromosome XII where several resistance genes are found clustered. A third PCR-based marker, GP198F-1, derived from the original RFLP marker GP198, was linked to the late blight resistance QTL on chromosome III that explained 23.4% of the total phenotypic variation in this population. The three PCR-based markers were used to screen for their presence in the mapping population and high correlations between the presence of these markers and late blight resistance were found. Our results suggest that these markers may be useful for MAS of late blight resistance in potato breeding programs.Una población híbrida diploide de Solanum phureja x S. stenotomum que ha mostrado niveles relativamente altos de resistencia cuantitativa a tizón tardío se usó para desarrollar marcadores basados en la reacción en cadena de la polimerasa (PCR) para la selección asistida por marcadores (MAS) de resistencia a la enfermedad en el mejoramiento de papa. Dos marcadores de ADN polimórfico amplificado al azar (RAPD), el OPA03$_{576}$ y OPA17$_{559}$ fueron identificados como ligados a resistencia parcial. Se encontró que OPA03$_{576}$ estaba asociado en fase de acoplamiento, mientras OPAl7$_{559}$ mostró asociación en fase de repulsión a la resistencia. Estos dos marcadores, junto con los marcadores previamente desarrollados de fragmentos polimórficos de ADN amplificados (AFLP) y de secuencias simples repetidas (SSR) fueron incorporados a un mapa cromosómico de ligamiento previo basado en fragmentos de restricción polimórficos (RFLP) de esta población S. phureja x S.stenotomum. El análisis de ligamiento localizó al marcador OPA17$_{559}$ en el cromosoma III, a 12 cM de un locus de caracteres cuantitativos (QTL) importante que explicó el 23.4% del total de la variación fenotípica para la resistencia a tizón tardío. El marcador OPA03$_{576}$ fue localizado en la región distal del cromosoma XII donde se encuentran agrupados varios genes de resistencia. Un tercer grupo de marcadores con base de PCR, el GP198F-1, derivado del marcador RFLP original GP198, fue ligado al QTL de resistencia al tizón tardío en el cromosoma III, que explicó el 23.4% de la variación total fenotípica en esta población. Los tres marcadores basados en PCR fueron usados para la detección de su presencia en la población de mapeo y se encontró una alta correlación entre la presencia de estos marcadores y la resistencia al tizón tardío. Nuestros resultados sugieren que estos marcadores pueden ser útiles en el MAS para resistencia a tizón tardío en los programas de mejoramiento de papa. |
| Starting Page | 188 |
| Ending Page | 195 |
| Page Count | 8 |
| File Format | |
| ISSN | 1099209X |
| Journal | American Potato Journal |
| Volume Number | 86 |
| Issue Number | 3 |
| e-ISSN | 18749380 |
| Language | English |
| Publisher | Springer-Verlag |
| Publisher Date | 2009-02-26 |
| Publisher Institution | Potato Association of America |
| Publisher Place | New York |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Marker assisted selection Quantitative trait loci Phytophthora infestans Solanum tuberosum Plant Pathology Plant Breeding/Biotechnology Plant Genetics & Genomics Agriculture Plant Sciences |
| Content Type | Text |
| Resource Type | Article |
| Subject | Plant Science Agronomy and Crop Science |
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