NDLI: Selection and Validation of an AFLP Marker Core Collection for the Wild Potato Solanum microdontum

Content Provider	Springer Nature Link
Author	Bamberg, John Rio, Alfonso
Copyright Year	2013
Abstract	Solanum microdontum is a diploid potato species with features that make it a good model for research into management and use of germplasm in the genebank. Its taxonomic status is unambiguous and it is in the taxonomic series of wild species closest to cultivated forms. It is represented by about 100 populations in the genebank—not too many for comprehensive evaluation, yet not too few to make prioritization of the most valuable populations worthwhile. This species is also particularly rich in desirable traits, often exhibiting very broad segregation. We here report use of DNA markers for selection of a core set of populations, and assessing whether that core captures populations with the most desirable evaluation results for economic traits. DNA was extracted from bulks of 27 plants from each of 94 populations to generate AFLPs. A total of 1,741 informative loci were detected. AFLP loci were treated as though they were traits, with the banded condition considered to be the desired state to include in a core set. At least one band unique to a population was present in 45 populations, and these 45 populations together captured 98 % of all bands. Adding another 14 populations for a core of 59 populations captured 100 % of bands. This core set was assessed for whether it encompassed those populations known to have useful traits, including nutritional and quality components; and disease, stress and pest resistances. As with AFLP bands, 25 of 26 of the most desirable phenotypic traits were also found in populations in the core set of 59 populations. The most desirable status of 3 traits is lost by selecting a core of 45 populations. We conclude that these core sets would be a rational starting point when prospecting for new useful traits in microdontum. Solanum microdontum es una especie diploide de papa con características que la hacen un buen modelo para investigación en manejo y uso de germoplasma en el banco de germoplasma. Su situación taxonómica es clara y es la serie taxonómica de especies silvestres más cercanas a las formas cultivadas. Esta representada por cerca de 100 poblaciones en el banco de germoplasma, no son muchas para evaluación entendible, pero tampoco son pocas para hacer priorización de las poblaciones más valiosas que valgan la pena. Esta especie también es particularmente rica en caracteres deseables, exhibiendo a menudo una amplia segregación. Aquí reportamos el uso de marcadores de ADN para la selección de un juego medular de poblaciones, y para analizar si ese grupo captura poblaciones con el resultado de la evaluación más deseable para caracteres económicos. Se extrajo el ADN de grupos de 27 plantas de cada una de 94 poblaciones para generar AFLPs. Se detectó un total de 1741 loci informativos. Estos fueron tratados como si fueran caracteres, con la condición de bandas consideradas como el estado deseable para incluirse en un grupo medular. Por lo menos una banda única a una población estuvo presente en 45 poblaciones, y éstas juntas capturaron 98 % de todas las bandas. Agregándole otras 14 poblaciones para un núcleo de 59 poblaciones capturaron el 100 % de las bandas. Se evaluó este juego medular para ver si abarcaba a aquellas poblaciones que se sabía que tenían caracteres útiles, incluyendo componentes nutricionales y de calidad; y resistencia a enfermedades, agobios y plagas. Con las bandas de AFLP, 25 de 26 de los caracteres fenotípicos más deseables también se encontraron en poblaciones en el juego medular de 59 poblaciones. La situación más deseable de tres caracteres se pierde mediante la selección de un grupo de 45 poblaciones. Concluimos que estos juegos o grupos medulares serían un punto inicial de partida racional cuando se les busquen nuevos caracteres útiles en microdontum.
Starting Page	368
Ending Page	375
Page Count	8
File Format	PDF
ISSN	1099209X
Journal	American Potato Journal
Volume Number	91
Issue Number	4
e-ISSN	18749380
Language	English
Publisher	Springer US
Publisher Date	2013-12-20
Publisher Institution	Potato Association of America
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Core Microdontum Genebank Plant Sciences Agriculture Plant Genetics & Genomics Plant Breeding/Biotechnology Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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