NDLI: Detection and Differentiation of Potato Virus Y Strains by Melting Analysis of an Oligonucleotide Virus Probe

Content Provider	Springer Nature Link
Author	Rotem, N. Shtein, C. Rosner, A. Levy, D. Rabiwitch, H. D.
Copyright Year	2016
Abstract	A novel simple, fast qualitative method for detection and differentiation of PVY strains in potatoes by the LightCycler technology is described. Fluorescent-labeled probe designed to contain variable degree of homology with a ‘target sequence’ of several known reference PVY strains was annealed to PCR products of these viruses followed by a graded melting analysis. The specific characteristics of the melting curves enable the detection, distinction and differentiation of each of following four known PVY strains O-FL, O-RB, N and NTN, in a single reaction obviating the need for size or nucleotide sequence analyses. In addition, it was demonstrated that virus extracts from plants infected with more than one strain can be resolved using this procedure. The melting-curves of extracts from 55 market size tubers harvested randomly from commercial fields and compared with those of the reference virus strains, revealed the presence of O-FL, O-RB and NTN strains of PVY.Se describe un método novedoso, simple, cualitativo, rápido, para la detección y diferenciación de variantes de PVY en papa por la tecnología de LightCycler. Se templó una sonda etiquetada fluorescente designada para contener un grado variable de homología con una “secuencia-objetivo” de varias líneas de PVY conocidas como referencia, para productos de PCR de estos virus, seguida por un análisis de graduación de disolución. Las características específicas de las curvas de disolución habilitan la detección, distinción y diferenciación de cada una de las siguientes cuatro variantes conocidas de PVY, O-FL, O-RB, N y NTN, en una sola reacción, obviando la necesidad de análisis del tamaño o de secuencia de nucleótidos. Además, se demostró que los extractos de virus de plantas infectadas con más de una variante se pueden determinar usando este procedimiento. Las curvas de fusión de extractos de 55 tubérculos de tamaño comercial cosechados al azar de campos comerciales y comparados con los de las variantes de los virus de referencia, revelaron la presencia de las variantes de PVY O-FL, O-RB y NTN.
Starting Page	620
Ending Page	625
Page Count	6
File Format	PDF
ISSN	1099209X
Journal	American Potato Journal
Volume Number	93
Issue Number	6
e-ISSN	18749380
Language	English
Publisher	Springer US
Publisher Date	2016-08-08
Publisher Institution	Potato Association of America
Publisher Place	New York
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	PVY PCR RT-PCR Molecular analysis Genotyping Plant Sciences Agriculture Plant Genetics & Genomics Plant Breeding/Biotechnology Plant Pathology
Content Type	Text
Resource Type	Article
Subject	Plant Science Agronomy and Crop Science

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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Distribution of Potato virus Y Strains in Tubers during the Post-Harvest Period

Mechanical Transmission of Potato Virus Y (PVY) Through Seed Cutting and Plant Wounding

Detection and Differentiation of Potato Virus Y Strains from Potato Using Immunocapture Multiplex RT-PCR

The Adaptation of Silica Capture RT-PCR for the Detection of Potato Virus Y

Genetic Diversity of Potato virus Y Complex

Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers

Studies on varietal response to different strains of Potato virus Y (PVY) reveal hypersensitive resistance in Exploits to PVY$^{O}$ and extreme resistance in F87084 to all tested strains

Crop Management Practices and Reduction of On-Farm Spread of Potato virus Y: a 5-Year Study in Commercial Potato Fields in New Brunswick, Canada

Introduction to 2009 International Symposium on Potato Virus Y

Detection and Differentiation of Potato Virus Y Strains by Melting Analysis of an Oligonucleotide Virus Probe

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Distribution of Potato virus Y Strains in Tubers during the Post-Harvest Period

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Optimization of a Real-Time RT-PCR Assay and its Comparison with ELISA, Conventional RT-PCR and the Grow-out Test for Large Scale Diagnosis of Potato virus Y in Dormant Potato Tubers

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