NDLI: Mosaic structure of the small cryptic plasmid pKST23 from Escherichia coli

Content Provider	Springer Nature Link
Author	Fecskeová, L. Kovařík, M. Javorský, P. Pristaš, P.
Copyright Year	2012
Abstract	Small plasmid pKST23 was isolated from sheep ruminal Escherichia coli population. Plasmid sequence was determined to be 2,779 bp in length and was found to have an overall 42 % of GC pairs. However, its sequence can be divided into two regions based on genetic composition and the GC content. It was found that the high GC region spanning approximately from nucleotide 1,300 to 2,750 was identical to a group of small Escherichia coli plasmids and encoded a putative replication protein identical to plasmid pKL1 Rep protein. The part with lower GC pairs seemed to be more specific as it showed no similarity to the GenBank database. Computational analysis revealed four open reading frames, two of which showed considerable homology to replication proteins. PCR primers targeting parts of the two different regions of plasmid pKST23 were used to assess the occurrence of related plasmids within ruminal E. coli population.
Starting Page	277
Ending Page	279
Page Count	3
File Format	PDF
ISSN	00155632
Journal	Folia Microbiologica
Volume Number	57
Issue Number	4
e-ISSN	18749356
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-06-02
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Applied Microbiology Environmental Engineering/Biotechnology Immunology Life Sciences Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology

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