NDLI: Evaluation of strains derived fromEscherichia coli W as hosts for the expression of penicillin G-acylase-encoding gene cloned on the recombinant plasmid pKA18

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Evaluation of strains derived fromEscherichia coli W as hosts for the expression of penicillin G-acylase-encoding gene cloned on the recombinant plasmid pKA18

Content Provider	Springer Nature Link
Author	Grafková, J. Sobotková, L. Kyslík, P.
Copyright Year	2002
Abstract	The potential for production of penicillin G-acylase (PGA), encoded by the chromosomal genepga $^{i}$, of four strains belonging to a genealogical line derived from the strainEscherichia coli W, was evaluated in a medium with and without the inducer phenylacetic acid (PA). These strains were used as hosts of the recombinant plasmid pKA18, in which the structural genepga $^{c}$ isolated from the strain RE3, the best host strain of a line giving the highest production, was cloned. The presence of the inducer reduced the copy number of the plasmid in all recombinant strains. Only in recombinant strain RE3 (pKA18) the reduction of the gene dosage ofpga $^{c}$ resulted also in the reduction of the amount of PGA synthesized by the cells. The reduced activity of the cells did not result from a segregation of plasmid-free clones. Also the growth rate was decreased by 20 and 40% in the host and recombinant strains, respectively. The host strain RE3 showing the highest production of PGA was also the best host of the recombinant plasmid in terms of the segregational stability and copy number (198 copies per chromosome). The recombinant strain RE3 (pKA18) also provided the highest production of PGA.
Starting Page	189
Ending Page	192
Page Count	4
File Format	PDF
ISSN	00155632
Journal	Folia Microbiologica
Volume Number	47
Issue Number	2
e-ISSN	18749356
Language	English
Publisher	Springer Netherlands
Publisher Date	2002-01-01
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Life Sciences Microbiology Applied Microbiology Immunology Environmental Engineering/Biotechnology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology

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