NDLI: Biochemical and molecular characterization of wild-type and fused protoplasts of Beauveria bassiana and Metarhizium anisopliae

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Biochemical and molecular characterization of wild-type and fused protoplasts of Beauveria bassiana and Metarhizium anisopliae

Content Provider	Springer Nature Link
Author	Ranga, V. Saini, Gurvinder Kaur
Copyright Year	2011
Abstract	Protoplasts were isolated from two isolates each of Beauveria bassiana and Metarhizium anisopliae using lysing enzymes. Intra- and intergeneric protoplast fusion has been carried out using 40% polyethylene glycol. The fused protoplasts of B. bassiana and M. anisopliae have been regenerated on Czapek–Dox agar media, and a total of four fusants were selected for further studies. An increase in proteinase and chitinase enzyme activity was recorded with all fusants as compared to the wild-type isolates. To understand the nature of recombination process, random amplification of polymorphic DNA (RAPD) and restriction fragment length polymorphism (RFLP) were carried out on genomic DNA of fused and wild-type isolates. The present study demonstrates the scope and significance of the protoplast fusion technique as a rapid consistent method for identification of B. bassiana and M. anisopliae fused and wild-type isolates based on the banding pattern of RAPD and RFLP that can be reliably used ahead for further applications on these species.
Starting Page	289
Ending Page	295
Page Count	7
File Format	PDF
ISSN	00155632
Journal	Folia Microbiologica
Volume Number	56
Issue Number	4
e-ISSN	18749356
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-08-05
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Environmental Engineering/Biotechnology Life Sciences Microbiology Applied Microbiology Immunology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology

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