NDLI: Differentiation of petroleum hydrocarbon-degrading Pseudomonas spp. based on PCR-RFLP of the 16S-23S rDNA intergenic spacer region

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Differentiation of petroleum hydrocarbon-degrading Pseudomonas spp. based on PCR-RFLP of the 16S-23S rDNA intergenic spacer region

Content Provider	Springer Nature Link
Author	Tanti, Bhaben Ray, Suvendra Kumar Buragohain, Alak Kumar
Copyright Year	2011
Abstract	Genetic diversity among 43 petroleum hydrocarbon-degrading Pseudomonas belonging to four different species and the type strain Pseudomonas aeruginosa MTCC1034 was assessed by using restriction fragment length polymorphism (RFLP) of polymerase chain reaction (PCR)-amplified 16S–23S rDNA intergenic spacer regions (ISRs) polymorphism. PCR amplification from all Pseudomonas species yielded almost identical ISR amplicons of “?” 800 bp and in nested PCR of “?” 550 bp. The RFLP analysis with MboI and AluI revealed considerable intraspecific variations within the Pseudomonas species. The dendrogram constructed on the basis of the PCR-RFLP patterns of 16S–23S rDNA intergenic spacer regions differentiated all the species into seven different clusters.
Starting Page	47
Ending Page	52
Page Count	6
File Format	PDF
ISSN	00155632
Journal	Folia Microbiologica
Volume Number	57
Issue Number	1
e-ISSN	18749356
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-12-23
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Environmental Engineering/Biotechnology Immunology Applied Microbiology Life Sciences Microbiology
Content Type	Text
Resource Type	Article
Subject	Medicine Microbiology

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