NDLI: Study on the stability and reusability of Glutamate Dehydrogenase immobilized on bacterial cellulose nanofiber

Content Provider	Springer Nature Link
Author	Pesaran, M. Amoabediny, Gh.
Copyright Year	2017
Abstract	In this study, a simple method with improved properties for Glutamate Dehydrogenase immobilization is proposed by using bacterial cellulosic nanofiberas as a novel and inexpensive biomatrix. Acetobacter xylinum ATCC 10245 has been chosen and cultivated for synthesis of this nanofiber with the diameter of 30-70 nm. Thereafter, Glutamate Dehydrogenase was immobilized into the nanofiber pores in two steps: 1) enzyme adsorption and 2) crosslinking by glutaraldehyde. Optimum pH for the activity of immobilized enzyme and optimum temperature was reported in this work. Also the relative activity of immobilized enzyme was about 38 % in comparison to the enzyme activity of the first day after about two months. Moreover after reusing of the enzyme for 9 cycles, the immobilized enzyme retained over 83 % of its initial activity. Glutamate Dehydrogenase is immobilized on bacterial cellulosic nanofiber for the first time which can affect on facilitation of Glutamate production. Also can be a good method for having a stable and robust immobilized Glutamate Dehydrogenase that can withstand harsh operating conditions in processes which are common in these kind of reactions.
Starting Page	240
Ending Page	245
Page Count	6
File Format	PDF
ISSN	12299197
Journal	Fibers and Polymers
Volume Number	18
Issue Number	2
e-ISSN	18750052
Language	English
Publisher	The Korean Fiber Society
Publisher Date	2017-02-28
Publisher Place	Seoul
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Bacterial cellulosic nanofiber Crosslinking Enzyme immobilization Operational stability Glutamate dehydrogenase Polymer Sciences
Content Type	Text
Resource Type	Article
Subject	Chemistry Chemical Engineering Polymers and Plastics

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