NDLI: Real-Time Micrography of Mouse Preimplantation Embryos in an Orbit Module on SJ-8 Satellite

Content Provider	Springer Nature Link
Author	Ma, Bao Hua Cao, Yu Jing Zheng, Wei Bo Lu, Jin Ren Kuang, Hai bin Lei, Xiao Hua Lv, Yin Huan Zhang, Tao Duan, En Kui
Copyright Year	2008
Abstract	The developmental capacity of mouse embryos in the Chinese SJ-8 Satellite was observed by real time micrography and telecontrol image transmission. Frozen/thawed 4-cell embryos and blastocysts injected with mouse epidemical stem cells were placed in a specially sealed embryonic incubator, and then the incubator was loaded in a space embryonic culture box devised for space-flight. After the satellite launched and arrived at the anticipated orbit, the real time micrography device was opened based on the telecontrol operational technology. Real time micrographs of the mouse embryos were obtained and stored every 3 hours, then the data of images were transmitted at the suitable time. The experiment persisted for 72 hours. The results showed that during space-flight, most mouse embryos cultured in the sealed culture unit kept integrity and natural structure, their location had minor change, but the embryos did not develop. However, the experiment performed on the ground in the same device showed that 4-cell mouse embryos could develop to blastocysts and hatched blastocysts. It may be concluded that the space environment, especially the change of gravity was likely to harm development of the mouse embryo.
Starting Page	127
Ending Page	136
Page Count	10
File Format	PDF
ISSN	09380108
Journal	Microgravity Science and Technology
Volume Number	20
Issue Number	2
e-ISSN	18750494
Language	English
Publisher	Springer Netherlands
Publisher Date	2008-05-30
Publisher Place	Dordrecht
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Space-flight Microgravity Satellite Real-time micrography Image transmission Embryonic development Mouse Mechanics, Fluids, Thermodynamics Extraterrestrial Physics, Space Sciences Space Exploration and Astronautics
Content Type	Text
Resource Type	Article
Subject	Applied Mathematics Physics and Astronomy Engineering Modeling and Simulation

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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