NDLI: Caco-2 cells infected with rotavirus release extracellular vesicles that express markers of apoptotic bodies and exosomes

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Caco-2 cells infected with rotavirus release extracellular vesicles that express markers of apoptotic bodies and exosomes

Content Provider	Springer Nature Link
Author	Bautista, Diana Rodríguez, Luz Stella Franco, Manuel A. Angel, Juana Barreto, Alfonso
Copyright Year	2015
Abstract	Previously, we showed that infecting human intestinal epithelial cells (Caco-2) with rotavirus (RV) increases the release of extracellular vesicles (EVs) with an immunomodulatory function that, upon concentration at 100,000×g, present buoyant densities on a sucrose gradient of between 1.10 to 1.18 g/ml (characteristic of exosomes) and higher than 1.24 g/ml (proposed for apoptotic bodies). The effect of cellular death induced by RV on the composition of these EV is unknown. Here, we evaluated exosome (CD63, Hsc70, and AChE) and apoptotic body (histone H3) markers in EVs isolated by differential centrifugation (4000×g, 10,000×g, and 100,000×g) or filtration/ultracentrifugation (100,000×g) protocols. When we infected cells in the presence of caspase inhibitors, Hsc70 and AChE diminished in EVs obtained at 100,000×g, but not in EVs obtained at 4000×g or 10,000×g. In addition, caspase inhibitors decreased CD63 and AChE in vesicles with low and high buoyant densities. Without caspase inhibitors, RV infection increased exosome markers in all of the EVs obtained by differential centrifugation. However, CD63 preferentially localized in the 100,000×g fraction and H3 only increased in EVs concentrated at 100,000×g and with high buoyant densities on a sucrose gradient. Thus, RV infection increases the release of EVs that, upon concentration at 100,000×g, are composed by exosomes and apoptotic bodies, which can partially be separated using sucrose gradients.
Starting Page	697
Ending Page	708
Page Count	12
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	20
Issue Number	4
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2015-05-15
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Extracellular vesicles Exosomes Apoptotic bodies Caco-2 Rotavirus Histone 3 Biomedicine general Cell Biology Biochemistry Immunology Cancer Research Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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