NDLI: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

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Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

Erratum to: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

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Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

Content Provider	Springer Nature Link
Author	Jing, Zhe Gangalum, Rajendra K. Lee, Josh Z. Mock, Dennis Bhat, Suraj P.
Copyright Year	2012
Abstract	Epithelial cells and fibroblasts both express heat shock transcription factors, HSF1 and HSF4, yet they respond to heat shock differentially. For example, while HSP70 is induced in both cell types, the small heat shock protein, αB-crystallin gene (CRYAB) that contains a canonical heat shock promoter, is only induced in fibroblasts. A canonical heat shock promoter contains three or more inverted repeats of the pentanucleotide 5′-nGAAn-3′ that make the heat shock element. It is known that, in vitro, promoter architecture (the order and spacing of these repeats) impacts the interaction of various heat shock transcription factors (HSFs) with the heat shock promoter, but in vivo relevance of these binding preferences so far as the expression is concerned is poorly understood. In this report, we first establish cell-type-dependent differential expression of CRYAB in four established cell lines and then working with adult human retinal pigment epithelial cells and NIH3T3 fibroblasts and employing chromatin immunoprecipitation, attempt to relate expression to promoter occupancy by HSF1 and HSF4. We show that HSF4 occupies only CRYAB and not HSP70 promoter in epithelial cells, while HSF1 occupies only HSP70 promoter in both cell types, and cryab promoter, only in heat shocked fibroblasts; HSF4, on the other hand, is never seen on these two promoters in NIH3T3 fibroblasts. This comparative analysis with CRYAB and HSP70 demonstrates that differential heat shock response is controlled by cell-type-dependent access of HSFs (HSF1 and HSF4) to specific promoters, independent of the promoter architecture.
Starting Page	377
Ending Page	387
Page Count	11
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	18
Issue Number	3
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2012-12-23
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Heat shock promoter HSF1 HSF4 Cell-type specific HSP70 αB-crystallin Biomedicine general Cell Biology Biochemistry Immunology Cancer Research Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock.

Erratum to: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

Erratum to: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

Erratum to: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

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Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

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Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock.

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Erratum to: Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock

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Cell-type-dependent access of HSF1 and HSF4 to αB-crystallin promoter during heat shock