NDLI: The role of Hsp27 and actin in the regulation of movement in human cancer cells responding to heat shock

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The role of Hsp27 and actin in the regulation of movement in human cancer cells responding to heat shock

Content Provider	Springer Nature Link
Author	Doshi, Bindi M. Hightower, Lawrence E. Lee, Juliet
Copyright Year	2009
Abstract	Human heat shock 27-kDa protein 1 (HSPB1)/heat shock protein (Hsp) 27 is a small heat shock protein which is thought to have several roles within the cell. One of these roles includes regulating actin filament dynamics in cell movement, since Hsp27 has previously been found to inhibit actin polymerization in vitro. In this study, the role of Hsp27 in regulating actin filament dynamics is further investigated. Hsp27 protein levels were reduced using siRNA in SW480 cells, a human colon cancer cell line. An in vitro wound closure assay showed that cells with knocked down Hsp27 levels were unable to close wounds, indicating that this protein is involved in regulating cell motility. Immunoprecipitation pull down assays were done, to observe if and when Hsp27 and actin are in the same complex within the cell, before and after heat shock. At all time points tested, Hsp27 and actin were present in the same cell lysate fraction. Lastly, indirect immunostaining was done before and after heat shock to evaluate Hsp27 and actin interaction in cells. Hsp27 and actin showed colocalization before heat shock, little association 3 h after heat shock, and increased association 24 h after heat shock. Cytoprotection was observed as early as 3 h after heat shock, yet cells were still able to move. These results show that Hsp27 and actin are in the same complex in cells and that Hsp27 is important for cell motility.
Starting Page	445
Ending Page	457
Page Count	13
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	14
Issue Number	5
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-02-18
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Hsp27 Actin Cell motility Heat shock Cancer Cancer Research Immunology Biochemistry Cell Biology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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The role of Hsp27 and actin in the regulation of movement in human cancer cells responding to heat shock.

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The role of Hsp27 and actin in the regulation of movement in human cancer cells responding to heat shock