NDLI: Effect of low oxygen tension on the biological characteristics of human bone marrow mesenchymal stem cells

Content Provider	Springer Nature Link
Author	Kim, Dae Seong Ko, Young Jong Lee, Myoung Woo Park, Hyun Jin Park, Yoo Jin Kim, Dong Ik Sung, Ki Woong Koo, Hong Hoe Yoo, Keon Hee
Copyright Year	2016
Abstract	Culture of mesenchymal stem cells (MSCs) under ambient conditions does not replicate the low oxygen environment of normal physiological or pathological states and can result in cellular impairment during culture. To overcome these limitations, we explored the effect of hypoxia (1 % O$_{2}$) on the biological characteristics of MSCs over the course of different culture periods. The following biological characteristics were examined in human bone marrow-derived MSCs cultured under hypoxia for 8 weeks: proliferation rate, morphology, cell size, senescence, immunophenotypic characteristics, and the expression levels of stemness-associated factors and cytokine and chemokine genes. MSCs cultured under hypoxia for approximately 2 weeks showed increased proliferation and viability. During long-term culture, hypoxia delayed phenotypic changes in MSCs, such as increased cell volume, altered morphology, and the expression of senescence-associated-β-gal, without altering their characteristic immunophenotypic characteristics. Furthermore, hypoxia increased the expression of stemness and chemokine-related genes, including OCT4 and CXCR7, and did not decrease the expression of KLF4, C-MYC, CCL2, CXCL9, CXCL10, and CXCR4 compared with levels in cells cultured under normoxia. In conclusion, low oxygen tension improved the biological characteristics of MSCs during ex vivo expansion. These data suggest that hypoxic culture could be a useful method for increasing the efficacy of MSC cell therapies.
Starting Page	1089
Ending Page	1099
Page Count	11
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	21
Issue Number	6
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2016-08-26
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Mesenchymal stem cell Hypoxia Biological characteristics Cell therapy Biomedicine Cell Biology Biochemistry Immunology Cancer Research Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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