NDLI: Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Please wait, while we are loading the content...

Ferruccio Ritossa’s scientific legacy 50 years after his discovery of the heat shock response: a new view of biology, a new society, and a new journal

Phage display biopanning identifies the translation initiation and elongation factors (IF1α-3 and eIF-3) as components of Hsp70–peptide complexes in breast tumour cells

Heterooligomeric complexes of human small heat shock proteins

The identification and characterization of IbpA, a novel α-crystallin-type heat shock protein from mycoplasma

Adenovirus-mediated expression of the HO-1 protein within MSCs decreased cytotoxicity and inhibited apoptosis induced by oxidative stresses

Characterisation of the Plasmodium falciparum Hsp70–Hsp90 organising protein (PfHop)

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Comparison of epitope specificity of anti-heat shock protein 60/65 IgG type antibodies in the sera of healthy subjects, patients with coronary heart disease and inflammatory bowel disease

Molecular and functional characterization of the only known hemiascomycete ortholog of the carboxyl terminus of Hsc70-interacting protein CHIP in the yeast Yarrowia lipolytica

OsHsfA2c and OsHsfB4b are involved in the transcriptional regulation of cytoplasmic OsClpB (Hsp100) gene in rice (Oryza sativa L.)

Immunomodulatory effect of NSAID in geriatric patients with acute infection: effects of piroxicam on chemokine/cytokine secretion patterns and levels of heat shock proteins. A double-blind randomized controlled trial. (ISRCTN58517443)

Prevention and treatment of alopecia areata with quercetin in the C3H/HeJ mouse model

A quantitative method for detection of spliced X-box binding protein-1 (XBP1) mRNA as a measure of endoplasmic reticulum (ER) stress

Cell Stress and Chaperones : Volume 17, Issue 1, January 2012

Cell Stress and Chaperones : Volume 16

Cell Stress and Chaperones : Volume 15

Cell Stress and Chaperones : Volume 14

Cell Stress and Chaperones : Volume 13

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Content Provider	Springer Nature Link
Author	Chung, Eunna Rylander, Marissa Nichole
Copyright Year	2011
Abstract	Conditioning protocols involving mechanical stress independently or with chemical cues such as growth factors (GFs) possess significant potential to enhance bone regeneration. However, utilization of thermal stress conditioning alone or with GFs for bone therapy has been under-investigated. In this study, a preosteoblast cell line (MC3T3-E1) was exposed to treatment with water bath heating (44°C, 4 and 8 min) and osteoinductive GFs (bone morphogenetic protein-2 and transforming growth factor-β1) individually or in combination to investigate whether these stimuli could promote induction of bone-related markers, an angiogenic factor, and heat shock proteins (HSPs). Cells remained viable when heating durations were less than 20 min at 40ºC, 16 min at 42ºC, and 10 min at 44ºC. Increasing heating duration at 44°C, promoted gene expression of HSPs, osteocalcin (OCN), and osteopontin (OPN) at 8 h post-heating (PH). Heating in combination with GFs caused the greatest gene induction of osteoprotegerin (OPG; 6.9- and 1.6-fold induction compared to sham-treated and GF only treated groups, respectively) and vascular endothelial growth factor (VEGF; 16.0- and 1.6-fold compared to sham and GF-only treated groups, respectively) at 8 h PH. Both heating and GFs independently suppressed the matrix metalloproteinase-9 (MMP-9) gene. GF treatment caused a more significant decrease in MMP-9 protein secretion to non-detectable levels compared to heating alone at 72 h PH. Secretion of OCN, OPN, and OPG increased with the addition of GFs but diminished with heating as measured by ELISA at 72 h PH. These results suggest that conditioning protocols utilizing heating and GFs individually or in combination can induce HSPs, bone-related proteins, and VEGF while also causing downregulation of osteoclastic activity, potentially providing a promising bone therapeutic strategy.
Starting Page	203
Ending Page	214
Page Count	12
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	17
Issue Number	2
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2011-11-25
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Bone healing Heating Heat shock protein Growth factors Preosteoblasts Neurosciences Biomedicine general Cancer Research Cell Biology Biochemistry Immunology
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors.

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Heat shock in the springtime

Characterization of rice small heat shock proteins targeted to different cellular organelles

Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons

Serum levels of heat shock protein 27 in patients with acute ischemic stroke

Regulation of survival gene hsp70

Molecular cloning of heat shock protein 60 (PtHSP60) from Portunus trituberculatus and its expression response to salinity stress

The heat shock response in congeneric land snails (Sphincterochila) from different habitats

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Similar Documents

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors.

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors

Heat shock in the springtime

Characterization of rice small heat shock proteins targeted to different cellular organelles

Reaction of small heat-shock proteins to different kinds of cellular stress in cultured rat hippocampal neurons

Serum levels of heat shock protein 27 in patients with acute ischemic stroke

Regulation of survival gene hsp70

Molecular cloning of heat shock protein 60 (PtHSP60) from Portunus trituberculatus and its expression response to salinity stress

The heat shock response in congeneric land snails (Sphincterochila) from different habitats

Response of preosteoblasts to thermal stress conditioning and osteoinductive growth factors