NDLI: The ambiguous role of the Na$^{+}$–H$^{+}$ exchanger isoform 1 (NHE1) in leptin-induced oxidative stress in human monocytes

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The ambiguous role of the Na$^{+}$–H$^{+}$ exchanger isoform 1 (NHE1) in leptin-induced oxidative stress in human monocytes

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Cell Stress and Chaperones : Volume 14, Issue 5, September 2009

Cell Stress and Chaperones : Volume 14, Issue 4, July 2009

Cell Stress and Chaperones : Volume 14, Issue 3, May 2009

Cell Stress and Chaperones : Volume 14, Issue 2, March 2009

Cell Stress and Chaperones : Volume 14, Issue 1, January 2009

Cell Stress and Chaperones : Volume 13

The ambiguous role of the Na$^{+}$–H$^{+}$ exchanger isoform 1 (NHE1) in leptin-induced oxidative stress in human monocytes

Content Provider	Springer Nature Link
Author	Konstantinidis, Diamantis Paletas, Konstantis Koliakos, George Kaloyianni, Martha
Copyright Year	2009
Abstract	Leptin, a 16-kDa cytokine produced mainly by the adipose tissue, is known to increase energy expenditure while at the same time lowering food intake by acting directly on the hypothalamus. ObRb, the leptin receptor mostly involved in intracellular signaling, is expressed in a wide range of tissues, thus allowing leptin to affect a much broader diversity of biological processes. High concentrations of leptin are encountered in patients with hyperleptinemia, a condition which very often accompanies obesity and which is a direct result of leptin resistance. In the present study, moderate and high concentrations of leptin (16 and 160 ng/ml) were mostly utilized in order to investigate the role of this cytokine in oxidative stress levels in human monocytes. Leptin was found to increase oxidative species production as measured with 2′,7′-dichlorodihydrofluorescein diacetate (general marker of oxidative species, but not O 2 −. ) and dihydroethidium (marker of O 2 −. ). Surprisingly, it also augmented superoxide dismutase activity. Inhibition of the Na$^{+}$–H$^{+}$ exchanger isoform 1 (NHE1) also inhibited leptin-induced superoxide anion production but at the same time amplified leptin-induced production of other oxidative species. Signaling proteins such as phosphoinositide 3 kinase and conventional isoforms of protein kinase C (α-, β$_{i}$-, β$_{ii}$-), as well as NADPH oxidase, also participated in leptin signaling. Finally, leptin was found to increase glutathionylation levels of NHE1-bound heat shock protein 70 kDa (Hsp70) but not Hsp70 binding to NHE1.
Starting Page	591
Ending Page	601
Page Count	11
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	14
Issue Number	6
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2009-03-20
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Atherosclerosis Leptin Monocytes NHE1 Oxidative stress Signaling Cancer Research Immunology Biochemistry Cell Biology Biomedicine general
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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