NDLI: LEA proteins are involved in cyst desiccation resistance and other abiotic stresses in Azotobacter vinelandii

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LEA proteins are involved in cyst desiccation resistance and other abiotic stresses in Azotobacter vinelandii

Content Provider	Springer Nature Link
Author	Rodriguez Salazar, Julieta More, Soledad Espín, Guadalupe
Copyright Year	2017
Abstract	Late embryogenesis abundant (LEA) proteins constitute a large protein family that is closely associated with resistance to abiotic stresses in multiple organisms and protect cells against drought and other stresses. Azotobacter vinelandii is a soil bacterium that forms desiccation-resistant cysts. This bacterium possesses two genes, here named lea1 and lea2, coding for avLEA1 and avLEA2 proteins, both containing 20-mer motifs characteristic of eukaryotic plant LEA proteins. In this study, we found that disruption of the lea1 gene caused a loss of the cysts’ viability after 3 months of desiccation, whereas at 6 months, wild-type or lea2 mutant strain cysts remained viable. Vegetative cells of the lea1 mutant were more sensitive to osmotic stress; cysts developed by this mutant were also more sensitive to high temperatures than cysts or vegetative cells of the wild type or of the lea2 mutant. Expression of lea1 was induced several fold during encystment. In addition, the protective effects of these proteins were assessed in Escherichia coli cells. We found that E. coli cells overexpressing avLEA1 were more tolerant to salt stress than control cells; finally, in vitro analysis showed that avLEA1 protein was able to prevent the freeze thaw-induced inactivation of lactate dehydrogenase. In conclusion, avLEA1 is essential for the survival of A. vinelandii in dry conditions and for protection against hyper-osmolarity, two major stress factors that bacteria must cope with for survival in the environment. This is the first report on the role of bacterial LEA proteins on the resistance of cysts to desiccation.
Starting Page	397
Ending Page	408
Page Count	12
File Format	PDF
ISSN	13558145
Journal	Cell Stress and Chaperones
Volume Number	22
Issue Number	3
e-ISSN	14661268
Language	English
Publisher	Springer Netherlands
Publisher Date	2017-03-03
Publisher Place	Dordrecht
Access Restriction	Subscribed
Subject Keyword	Azotobacter LEA proteins Cysts desiccation Osmotic stress High temperatures Biomedicine Cell Biology Biochemistry Immunology Cancer Research Neurosciences
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry

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LEA proteins are involved in cyst desiccation resistance and other abiotic stresses in Azotobacter vinelandii.

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LEA proteins are involved in cyst desiccation resistance and other abiotic stresses in Azotobacter vinelandii

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